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A sensitive radiometric assay for ornithine aminotransferase: regional and subcellular distributions in rat brain


, : A sensitive radiometric assay for ornithine aminotransferase: regional and subcellular distributions in rat brain. Journal of Neurochemistry 36(2): 501-505

A radiometric assay for ornithine aminotransferase was developed using [1-14C]alpha-ketoglutarate as the labeled substrate and glutamate decarboxylation as a linking step. This assay gives near total measurement of ornithine aminotransferase activities that are, respectively, about 1.5 and 10 times larger than those obtained by the spectrophotometric assay and the radiometric assay using [1-14C]ornithine. It is also the most sensitive of the three assay procedures. Consistent with previous reports, brain ornithine aminotransferase was found to be present predominantly in synaptosomes. Regional distribution of the enzyme correlated with that of the high-affinity uptake of glutamate, but not with the distribution of glutamate decarboxylase. Ornithine aminotransferase may be responsible for the synthesis of glutamate in glutamatergic neurons but it is clearly not localized exclusively in such neurons.

US$29.90

PMID: 7007578


Other references

Rauch R.F.; Korobkin M.; Silverman P.M.; Moore A.V., 1983: Computed tomographic detection of iatrogenic per cutaneous splenic injury. Although the potential for splenic injury left sided thoracentesis or percutaneous renal biopsy is well known, its occurrence was rarely reported. In a 1 yr period computed tomography was used to detect acute splenic, perisplenic and intraperitone...

Leissinger, C.; Carcao, M.; Gill, J.C.; Journeycake, J.; Singleton, T.; Valentino, L., 2014: Desmopressin (DDAVP) in the management of patients with congenital bleeding disorders. Bleeding disorders, including haemophilia, von Willebrand disease, and platelet function abnormalities pose a substantial, ongoing management challenge. Patients with these disorders not only require treatment during bleeding events but also need...

Parveen, B.Ahamed.; Sindhuja, R., 2008: Rabson-Mendenhall syndrome. Rabson mendenhall syndrome is a rare autosomally recessive inherited insulin resistant disorder. This is characterized by growth retardation, dysmorphisms, lack of subcutaneous fat, acanthosis nigricans, enlarged genitalia hypertrichosis, prematur...

Woolley, K.; Martin, P., 2000: Conserved mechanisms of repair: from damaged single cells to wounds in multicellular tissues. The capacities to repair minor membrane holes in damaged single cells, and the more major damage sustained when a multicellular tissue is wounded, both involve a series of ancient and highly conserved processes. In this review, we discuss what is...

Heiner A.; Nilsson A., 1992: Stabilization of an open cut excavation at the Mrica hydropower project, Indonesia. Proceedings of the International Symposium on Landslides = Comptes Rendus du Symposium International sur les Glissements de Terrain 6: 743-750

Xambeu., 1894: Entomologie appliquee a la medecine. Soc Pyrenees-or, XXXV 145 & 146

Kutuk, M.S.; Goksedef, B.P.C., 2012: A postmenopausal woman developed a giant endometrial polyp during Raloxifene treatment. Journal of Obstetrics and Gynaecology 31(7): 672-672

Hoffmann V.; Fehr K.T., 1996: Micromagnetic, rockmagnetic and mineralogical studies on dacitic pumice from the Pinatubo eruption (1991, Philippines) showing self-reversed TRM. Geophysical Research Letters 23(20): 2835-2838

Eriksson, N.E.; Wihl, J.A.; Arrendal, H.; Strandhede, S.O., 1984: Tree pollen allergy 2. sensitization to various tree pollen allergens in sweden a multi center study. In a multi-center study, comprising 16 clinics, 871 adult hay fever patients were studied. Most patients included had springtime hay fever. Skin prick tests were performed with 20 different tree or bush pollen (lignoses) allergens. RAST [radioalle...

Patil, M.S.astri, N., 1982: Effect of various carbon and nitrogen sources on extracellular production of proteases by Alternaria alternata (Fr.) Keissl Fungi. Indian journal of microbiology 22(2): 115-122