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A sensitive staining method for detecting acidic polysaccharides in cellulose acetate and agarose gels


, : A sensitive staining method for detecting acidic polysaccharides in cellulose acetate and agarose gels. Analytical Biochemistry 130(1): 60-64

A sensitive staining method has been developed for the detection of acidic polysaccharides in cellulose acetate and agarose gels. The method is based on the precipitation of bovine serum albumin by acidic polysaccharides at acidic pH values and the subsequent staining of precipitated protein with amido black or Coomassie brilliant blue R-250 stains. The detection limit of acidic polysaccharides is 15-40 ng on cellulose acetate strips and 50-150 ng on agarose plates. The sensitivity of the described staining technique is of the same order for a wide range of acidic polysaccharides of different origin in contrast to Alcian blue and toluidine blue stains, which detect only mucopolysaccharides of animal origin at comparable levels. The method was also applied to the colorimetric quantitative determination of acidic polysaccharides after electrophoretic separation.

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PMID: 6191594


Other references

Dreborg, S.; Basomba, A.; Einarsson, R., 1986: Sensitivity to Parietaria officinalis and Parietaria judaica pollen allergens in a Spanish population. To investigate the biological activity of P. judaica and P. officinalis allergen preparations, 36 Spanish patients were included in a biological standardization (BS) trial. For both species the concentration corresponding to 1000 biological units/...

Akimoto, K.; Furutani, M.; Imamura, S.; Furutani, Y.; Kasanuki, H.; Takao, A.; Momma, K.; Matsuoka, R., 1998: Novel missense mutation (G601S) of HERG in a Japanese long QT syndrome family. Human Mutation Suppl 1: S184-S186

Forcina, G.; D'ettorre, G.; Corpolongo, A.; Santopadre, P.; Coletta, S.; Massetti, A.P.; Mastroianni, C.M.; Vullo, V., 2000: Interleukin-15 induces interleukin-8 and monocyte chemotactic protein-1 production in HIV-infected patients. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 40: 290

Babkin, B.P.; Bowie, D.J.; Nicholls, J.V.V., 1933: Structure and reactions to stimuli of arteries (and conus) in the Elasmobranch genus Raja. Contributions to Canadian Biology (NS): 8: 207-225

McKaye, K.R., 1986: Trophic eggs and parental foraging for young by the catfish Bagrus meridionalis of Lake Malawi, Africa. Parental bagrid catfish in Lake Malawi, Africa, appear to feed their young in a manner analogous to birds and social insects. The female produces eggs which are released and consumed by the catfish young. Indirect evidence suggests that the male l...

Alijotas-Reig, J.; Cistero-Bahima, A., 2004: Therapeutic management of antiphospholipid syndrome in aspirin sensitive patients. Lupus 13(7): 554-554

Bodeanu, N., 1984: Modifications sous l'influence anthropique dans le developpement quantitatif et dans la structure du phytoplancton du secteur roumain de la mer Noire. Travaux du Museum d'Histoire Naturelle "Grigore Antipa": 26: 69-83

Lusena, Marcello, 1926: Modifications of type of mutation induced in cultures of typhoid by treating with immune sera. A strain of Bacillus typhosus was studied which after repeated treatments with agglutinating serum of high titer became serum-resistant and in some instances took on the characteristics of B. paratyphosus B and became agglutinable by anti-paratyph...

E.S.idi, M.T.; Kuiper, P.J.C., 1972: Effect of applied kinetin on uptake and transport of 22Na and 36Cl in bean and cotton plants. Application of 0.1-1 mg/l kinetin to the roots of Phaseolus vulgaris cv. Widusa stimulated the transport of 22Na, applied to the leaf tip, from the leaf to the stem and roots. When roots of Gossypium hirsutum cv. Upland Long Staple were exposed to...

Radulovic, Vladan., 1993: Brachiopod zones in the Middle Jurassic of the Yugoslavian Carpatho-Balkanides. Geoloshki Anali Balkanskoga Poluostrva. Decembre; 572: 65-74