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A sensitive, continuous spectrophotometric method for assaying alpha-glycerophosphate dehydrogenase: activation by menadione


, : A sensitive, continuous spectrophotometric method for assaying alpha-glycerophosphate dehydrogenase: activation by menadione. Analytical Biochemistry 139(2): 319-321

By use of a more sensitive method than standard assays, it is demonstrated that alpha-glycerophosphate dehydrogenase activity can be monitored continuously in rat liver mitochondria; maximum activity was obtained by sonication in the presence of Triton X-100. Vitamin K3 (menadione) seems to enhance the activity of the enzyme. The assay in the presence of menadione is linear over a much greater mitochondrial concentration (up to 250 micrograms of protein in the reaction mixture).

US$29.90

PMID: 6476369


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Hirano, M.; Tomita, H.; Nagashima, T.; Matsushita, H.; Kawasaki, H., 1970: Paradoxical movement of the vocal cord in recurrent laryngeal nerve paralysis. Nihon Jibiinkoka Gakkai Kaiho 73(7): 885-891

Wolterstorff, W., 1905: Zur Biologie der Rana temporaria L. Zoologischer Anzeiger, XXVIII 536-538

Wasserman K., 1984: Estimating mean arterial carbon di oxide partial pressure from alveolar carbon di oxide profiles during exercise in man. Federation Proceedings: Abstract 785

Sun Y.; Deibler G.E.; Sokoloff L.; Smith C.B., 1992: Determination of regional rates of cerebral protein synthesis adjusted for regional differences in recycling of leucine derived from protein degradation into the precursor pool in conscious adult rats. The quantitative autoradiographic L-[1-14C]leucine method for the determination of regional rates of cerebral protein synthesis in vivo takes into account recycling of unlabeled leucine derived from protein degradation into the precursor pool for...

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Ueda, K.; Nakajima, H.; Nakagawa, T.; Shimizu, A., 1995: The association of complement activation at a low temperature with hepatitis C virus infection in comparison with cryoglobulin. Complement activation at a low temperature in vitro and cryoglobulinaemia are associated with hepatitis C virus (HCV) infection. The frequency of HCV antibody positivity determined in serum specimens that showed the cold-dependent activation of co...

Kerbikov, O.V., 1952: Certain disputable problems in psychiatry. Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova 52(5): 8-25