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A sensitive, single-radial diffusion autoradiographic zone size enhancement technique for the assay of influenza haemagglutinin


, : A sensitive, single-radial diffusion autoradiographic zone size enhancement technique for the assay of influenza haemagglutinin. Journal of General Virology 47(2): 355-363

A novel radial-diffusion technique in agarose gels is described which is applicable to the assay of small concentrations of influenza haemagglutinin (HA) antigen. The method is based on single-radial diffusion zone size enhancement (ZE) using autoradiography to demonstrate antigen--antibody reactions. The ZE technique is capable of reliably assaying concentrations of haemagglutinin as low as 0.1 microgram/ml, which represents a 20- to 40-fold increase in sensitivity over conventional single-radial-diffusion techniques. The ZE response is dependent upon the antigenic identity of the 125I-labelled 'marker' HA and the HA antigen being assayed. Haemagglutinins showing variation within a subtype (e.g. H3) show reduced ZE responses and thus the method has potential value for distinguishing between closely related HA antigens.

US$19.90

PMID: 6767808

DOI: 10.1099/0022-1317-47-2-355


Other references

Piatkowska, B., 1961: Effect of increased temperature and nutritive factors on the facet-number in the mutant-bar of Drosophila melanogaster Meig. The number of facets in the eye of Drosophila melanogaster Meig. were lowered with an increase in temperature. At 23 C the mean facet number for males was 86 and for females 92. At 30[degree] C the mean facet numbers were 38 and 43 for males and f...

Clauw, D.; Keeffe, B.; Maher, K.; Benjamin, S., 1996: Comparison of esophageal manometry and results of visceral nociceptive testing in individuals with fibromyalgia and noncardiac chest pain. Arthritis & Rheumatism 39(9 SUPPL ): S276

Grosveld F.; Antoniou M.; Blom G.; Catala F.; Collis P.; Deboer E.; Greaves D.; Hanscombe O.; Hurst J.; E.A., 1989: Regulation of the human beta globin gene in transgenic mice and cultured cells. Beaudet, A L , R Mulligan And I M Verma (Ed ) Ucla (University Of California Los Angeles) Symposia on Molecular And Cellular Biology New Series, Vol 87 Gene Transfer And Gene Therapy; Tamarron, Colorado, Usa, February 6-12, 1988 Xxii+441p Alan R Liss, Inc : New York, New York, Usa Illus 35-46

Naeimi Akbar, H.; Moharamzadeh, K.; Wood, D.J.; Van Noort, R., 2012: Relationship between Color and Translucency of Multishaded Dental Composite Resins. The aim of the present study was to compare the translucency of different shades of two highly aesthetic multilayered restorative composite resins. In total nine shades from Esthet.X and ten shades from Filtek Supreme composite resins were chosen....

Bilginturan, N., 1966: Congenital deafness and goiter (Pendred's syndrome). Turkish Journal of Pediatrics 8(4): 216-221

Gal, P.; Sharpless, M.K., 1987: Cromolyn sodium for childhood asthma: a drug whose time has come. Drug Intelligence & Clinical Pharmacy 21(1 Pt 1): 36-37

Singh, G.; Singh, D.P.; Gupta, D.; Muralikrishna, B.V., 1996: Neoadjuvant chemotherapy in locally advanced breast cancer. Thirty-eight patients with locally advanced breast cancer (Stage III) were treated over a 3-year period. All patients initially received two cycles of CMF (cyclophosphamide, 100 mg/m2 p.o. d1-14; methotrexate 40 mg/m2 intravenously (i.v.), d1 and...

Kengne, A.Pascal.; Czernichow, Sébastien.; Hamer, M.; Batty, G.David.; Stamatakis, E., 2013: Anaemia, haemoglobin level and cause-specific mortality in people with and without diabetes. Both anaemia and cardiovascular disease (CVD) are common in people with diabetes. While individually both characteristics are known to raise mortality risk, their combined influence has yet to be quantified. In this pooling project, we examined th...

Goksu, K., 1973: The occurrence of hydatidosis in black goats. Eighty-three (7.84%) male goats of 1,058 were found to be infected with hydatid in Antalya slaughterhouse in the southern part of Turkey. Hydatid cysts were found in the lungs of 76 and in the livers of 7.

Vivier, P.M.; Alario, A.J.; Simon, P.; Flanagan, P.; O'Haire, C.; Peter, G., 1999: Immunization status of children enrolled in a hospital-based medicaid managed care practice: the importance of the timing of vaccine administration. Objectives. To evaluate the immunization status of children enrolled in a hospital-based Medicaid managed care practice and to assess the impact of the timing of vaccine administration on measured immunization rates. Design and methods. The medica...