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A sensitive, specific radioisotope assay for L-glutamine-D-fructose-6-phosphate aminotransferase

, : A sensitive, specific radioisotope assay for L-glutamine-D-fructose-6-phosphate aminotransferase. Analytical Biochemistry 115(2): 347-352

A sensitive and specific radioassay for L-glutamine-D-fructose-6-phosphate aminotransferase (EC activity is presented; picomoles of product are measurable, and the assay can be applied to systems having limited quantities of available protein, particularly in extracts of either cell [human fibroblast] or organ cultures [rat liver, col, and ileum]. The assay is at least 10,000 times more sensitive under K1 concentrations of fructose 6-phosphate than the modified Elson-Morgan colorimetric assay and 20 times more sensitive under saturating conditions of fructose 6-phosphate. As little as 0.5 .mu.g of cell-extract protein will yield measurable product; 280 .mu.g of crude extract protein from colon is required with the modified Elson-Morgan colorimetric assay.


PMID: 7030130

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