geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A sensitive, specific, solid-phase enzymeimmunoassay for plasma progesterone


, : A sensitive, specific, solid-phase enzymeimmunoassay for plasma progesterone. Annals of Clinical Biochemistry 18(Pt 1): 42-47

A homologous enzymeimmunoassay (EIA) for plasma progesterone, using a horseradish peroxidase conjugate as enzyme label and an antiserum raised against a progesterone-11 kappa-hemisuccinyl/BSA conjugate, is described. The antiserum was covalently linked to microcrystalline cellulose to facilitate separation of bound and free steroid; this solid-phase antiserum was stable for at least nine months when stored at 4 degrees C. the freeze-dried enzyme label is also stable, having retained both enzymic and immunological activity for about four years. The EIA developed was specific and had the sensitivity (4.8 pg/tube) required for determining progesterone concentrations in in plasma samples collected at any time during the menstrual cycle. EIA of plasma samples provided results which were in good agreement with a well validated radioimmunoassay (RIA). The specificity and inter- and intra-assay coefficients of variation in the EIA were strictly comparable with those of the RIA. The method described has been in use for two years and has been assessed in external quality assurance programme established by the World Health organization and the United Kingdom Department of Health and Social Security.

US$29.90

PMID: 7020564


Other references

Geyman, M.J., 1929: Massive Pulmonary Atelectasis: Report Of Cases. California and Western Medicine 30(5): 351-353

Taylor, R., 1975: The Galway farmers. Although Ireland gains overall from the CAP and wants to remain in EEC, this does not overcome the Irish regional dilemma. Galway farmers are resistant to change. Many farmers do not produce any market produce at all. It is estimated that only 2 p...

Reading, R.P.; Vargas, A.; Miller, B.J.; Clark, T.W.; Hanebury, L.R.; Biggins, D., 1996: Recent directions in black-footed ferret recovery. Endangered Species Update. October-November; 1310-11: 1-6

Johnston, W.C., 1944: The use of superphosphate on pastures during rationing. Use of 45 lbs. superphosphate per acre per annum applied to subterranean clover increased the carrying capacity of the pasture 1.2 sheep per acre over the non-treated plot. 90 lbs. per acre increased the carrying capacity to 1.4 sheep and 180 lbs....

Hoffman J.I.E., 1973: Intra myo cardial distribution of coronary blood flow during hemorrhagic shock. Federation Proceedingspart 1: 403

Krystanov, S.F.lcheva, E., 1975: Adsorption of zinc in various soils of Bulgaria. Studies about Humus Transaction of the Internationalsymposium Humus et Planta: 123-130

Ball, N.; Haskell, M.J.; Deag, J.M.; Williams, J.L., 2002: Measuring temperament traits in cattle for QTL identification. Behavioural responses of cattle to handling and management are presumed to reflect underlying temperament traits such as fear. Individual variation in these behavioural responses has a genetic component, and hence information about the location of...

Liggett, Stephen B., 2004: Variation in drug response related to polymorphisms in beta2-adrenergic receptor. A method for predicting an individual's response to the beta-agonists salmeterol, albuterol, metaproterenol, terbutaline and formoterol is disclosed. Individuals expressing the Ile164 beta2 AR variant are likely to exhibit a reduced response...

Zakrzewski, T., 1954: Selected problems from practical neurology: paralysis of the facial nerve, short lasting unconsciousness. Wiadomosci Lekarskie 7(12): 623-627

Liu, M.; Lawson, G.; Delos, M.; Jamart, J.; Remacle, M., 1997: Evaluation of proliferating cell nuclear antigen (PCNA) in supraglottic carcinoma. Evaluation of proliferating cell nuclear antigen (PCNA) in supraglottic carcinoma. It is widely accepted that tumor invasion and metastasis are the primary causes for the lethal clinical outcome of cancers. In recent years, attention has been draw...