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A sequential quantitative study of the reversibility or irreversibility of liver hyperplastic nodules in rats exposed to hepatocarcinogens


, : A sequential quantitative study of the reversibility or irreversibility of liver hyperplastic nodules in rats exposed to hepatocarcinogens. Gan 71(6): 843-855

The behavior of hyperplastic nodules following an in vivo short-term screening test for hepatocarcinogens was studied. Rats were injected ip with 200 mg/kg body weight of diethylnitrosamine (DEN), given basal diet containing 200 ppm of N-2-fluorenylacetamide (2-FAA) (group 1), 1000 ppm of the alpha-isomer of 1,2,3,4,5,6,-hexachlorocyclohexane (alpha-BHC) (group 2) or basal diet (group 3) from week 3 to week 8, and then given basal diet and tap water. They were subjected to partial hepatectomy at the end of week 3. Animals were killed at weeks 4, 6, 8, 10, 20, 30, 40, and 50. A significant disappearance of hyperplastic nodules following the cessation of carcinogen treatment was observed in group 1, but was not evident in groups 2 and 3. With gamma-glutamyltranspeptidase (GGTase) as a positive marker and adenosine triphosphatase (ATPase) as a negative marker, hyperplastic nodules were classified into 3 different phenotypic categories, i.e., (1) GGTase-positive and ATPase-negative, (2) GGTase-positive, and (3) ATPase-negative. The percentages of GGTase-positive and ATPase-negative hyperplastic nodules were almost 80 approximately 90% in group 1 and 70 approximately 80% in groups 2 and 3. Some of the hyperplastic nodules were necrotic from week 8 in groups 1 and 2, and from week 20 in group 3. Subsequently, the numbers of necrotic hyperplastic nodules increased with time. Hepatocellular carcinomas were found at weeks 30, 40, and 50 in group 1, and at weeks 40 and 50 in group 2. Significantly higher incidences of cancer were found in group 1 than in group 2. The hepatocellular carcinomas were also classified enzyme-histochemically into 3 different phenotypic categories as for hyperplastic nodules, but the percentage (20%) of GGTase-positive and ATPase-negative hepatocellular carcinomas was significantly lower than that (70 approximately 90%) of GGTase-positive and ATPase-negative hyperplastic nodules in each group.

US$29.90

PMID: 6115791


Other references

Fukushima Yusuke; Hagihara Tatsuji; Sakamoto Mitsuo, 1998: Comparison of a powder snow avalanche model with thermal experiments on inclined boundaries. In this paper, the adequacy of numerical model of powder snow avalanches is examined by experiments of inclined thermals with depoisiting solid particles. The numerical model consists of mass conservation equations for the air and snow particles a...

Krause D.W., 1982: Multituberculates from the wasatchian land mammal age early eocene of western north america. Wasatchian multituberculates have been discovered in 5 major areas within the Western Interior of North America. Particularly large samples have been recovered from the Bighorn Basin of northwestern Wyoming [USA] and the Four Mile Creek area of no...

Alm O.; Roeshoff K.; Stephansson O., 1980: Microstructures and mechanical characteristics of the Taennaes augen gneiss, Swedish Caledonides. Geologiska Foereningen i Stockholm Foerhandlingar 102, Part 4: 319-334

Wang, T-fang., 2013: The establishment of diagnostic standards for pattern differentiation and disease identification. Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi 31(8): 1025-1026

Baril, G., 1931: The Correlation Of Laboratory Findings With The Clinical Aspects Of Disease. Canadian Medical Association Journal 24(4): 531-536

Y.H., 1986: A study on dextranase gene cloning of flavobacterium multivorum. Korean Journal Of Applied Microbiology & Bioengineering: 514

Durandy, A.; Taubenheim, N.; Peron, S.; Fischer, A., 2007: Pathophysiology of B-cell intrinsic immunoglobulin class switch recombination deficiencies. B-cell intrinsic immunoglobulin class switch recombination (Ig-CSR) deficiencies, previously termed hyper-IgM syndromes, are genetically determined conditions characterized by normal or elevated serum IgM levels and an absence or very low levels o...

Palacios-Prado, N.; Bukauskas, F.F., 2009: Heterotypic gap junction channels as voltage-sensitive valves for intercellular signaling. Gap junction (GJ) channels assembled from connexin (Cx) proteins provide a structural basis for direct electrical and metabolic cell-cell communication. By combining fluorescence imaging and dual whole-cell voltage clamp methods, we demonstrate th...

Nagai, S.; Ohara, K.; Mukai, K., 2006: Kinetic study of the quenching reaction of singlet oxygen by flavonoids in ethanol solution. The quenching rate of singlet oxygen (1O2) by seven kinds of flavonoids (flavone, flavonol, chrysin, apigenin, rutin, quercetin, and myricetin) with 2,3-double bonds has been measured spectrophotometrically in ethanol at 35 degrees C. The overall...

Dresnay Renaud du; Hupe Pierre, 1953: La serie cambrienne du Jebel Tibalicine (confins algero-marocains du sud). Descriptions of the stratigraphy and fauna of Cambrian formations of the Tibalicine range in southern Algeria near the Moroccan border.