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A series of hybrid cells containing different ratios of parental chromosomes formed by two steps of artificial fusion


, : A series of hybrid cells containing different ratios of parental chromosomes formed by two steps of artificial fusion. Proceedings of the National Academy of Sciences of the United States of America 68(1): 38-42

Double hybridization of Ehrlich ascites tumor cells (ETC) and L cells was performed. In the first step, a hybrid (LE) of ETC and L(AG) (a mutant of L cells, resistant to 10 mug/ml of 8-azaguanine) and a hybrid (LL) of L(AG) and L(BrdU) (a mutant of L cells, resistant to 100 mug/ml of 5-bromodeoxyuridine) were prepared by the use of artificial fusion by UV-irradiated HVJ (Sendai virus). In the second step the LE hybrid and ETC, or the LL hybrid and ETC, were fused again by UV-HVJ. The hybrids (LEE and LLE) were segregated during culture. Thus, the series of hybrids L, LLE, LE, LEE, and ETC was obtained. The morphological feature and karyological characters of these hybrids and the distribution of antigens corresponding to each parent on their cell surfaces supported the above identification of the series of hybrids obtained by double hybridization. All three hybrids acquired new characters, such as the ability to form large colonies in soft agar and large tumors on the chorioallantoic membrane of chicken eggs, unlike either parent. The tumor-forming capacity of the series was ETC > LEE > LE > LLE; L cells formed no tumor in mouse abdomen under the test conditions.

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PMID: 4322264


Other references

Oliver C., 1985: Secretion of acth and peptides correlated to obesity. Alimentazione Nutrizione Metabolismo: 202

L'vov; Procaccia, 1995: "Intermittency" in Hydrodynamic Turbulence as Intermediate Asymptotics to Kolmogorov Scaling. Physical Review Letters 74(14): 2690-2693

Pittard W.B.; Geddes K.M.; Brown S.; Mintz S.S.; Hulsey T.C., 1989: Bacterial contamination of expressed human milk. Pediatric Research 25(4 PART 2): 122A

Schnorr, Jörg.; Wagner, S.; Pilgrimm, H.; Hamm, B.; Taupitz, M., 2002: Preclinical characterization of monomer-stabilized very small superparamagnetic iron oxide particles (VSOP) as a blood pool contrast medium for MR angiography. Academic Radiology 9 Suppl 2: S307-S309

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Lindsay, Erin J., 2001: Blood treatment system. A blood treatment system has a housing formed of transparent plastic material configured so that substantially the entire blood flow path is visible. A generally planar blood filtration media assembly divides a blood treatment chamber within the h...

Tran, A.; Tréluyer, J.M.; Rey, E.; Barbet, J.; Ferracci, G.; d'Athis, P.; Vincent, J.; Pons, G., 2001: Protective effect of stiripentol on acetaminophen-induced hepatotoxicity in rat. Acetaminophen (APAP) is mainly eliminated at a therapeutic dose through glucuronidation and sulfatation and a small fraction is oxidized by cytochromes P450 (CYP) 2E1, 3A4, and 1A2 to N-acetyl-p-benzoquinone-imine (NAPQI), a highly reactive metabo...

Rutegård, J.N.; Janunger, K.G., 1991: Therapy-resistant destructive urinary tract infection of unknown origin: a case report. Infection 19(2): 123-123

Hadley, G.G.; Neilsen, I.R.; Clark, W.B., 1974: Can we predict which students will work overseas after graduation?. Medical Arts and Sciences 28(2): 18-23