geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A serine proteinase from Thermoactinomyces vulgaris, strain INMI-4a


, : A serine proteinase from Thermoactinomyces vulgaris, strain INMI-4a. Biokhimiia 45(10): 1871-1880

A serine proteinase was isolated from the cultural filtrate of the thermophylic actinomycet Thermoactinomycet vulgaris, strain INMI-4a. The purification procedure included affinity chromatography on bacitracin-Sepharose, ion-exchange separation on aminosilochrome, and gel-filtration on Sephadex G-25, resulting in a 194-fold purification and the 55% yield of the enzyme. The molecular weight of the enzyme as determined by polyacrylamide gel electrophoresis in the presence of Na-SDS as well as by gel-filtration on Sephadex G-75 is equal to 28 000; the amino acid composition is: Lys11, His4, Arg5, Asp33, Thr22, Ser24, Glu16, Pro16, Gly30, Ala38, Cys1-2, Val20, Met1, Ile14, Leu8, Tyr16, The4, Trp6-7. The isoelectric point lies at pH 8--9; the pH optimum for the peptide substrate hydrolysis is Z-L-Ala-L-Ala-L-Leu-pNA is at 8.2. The enzyme is stable at pH 7--9. The temperature optimum of the proteolytic activity lies at 55 degrees; however, the enzyme is stable to heating for 1 h at 37 degrees. The proteinase is completely inactivated by the serine proteinase specific inhibitors--phenylmethylsulphofluoride and the protein inhibitor IT-AjT from Actinomyces, as well as by p-chloromercuribenzoate. The enzyme shows lytic activity against the cells of E. coli, Micrococcus lysodeicticus and of the yeasts. The Thermoactinomyces vulgaris serine proteinase, being definitely different from the serine proteinases from Actinomyces griseus, also reveals specific differences when compared to bacterial serine proteinases, e. g. subtilisins. There are some indications to the enzyme relationship with the family of carboxypeptidase Y-like serine proteinases.

US$29.90

PMID: 7016198


Other references

Burstone, C.; Filleul, M.P.; Pigeot, V., 2000: Stability of orthodontic treatment of occlusal asymmetry. A common finding in orthodontic patients is asymmetric occlusion. These asymmetries can be dental, skeletal, or functional in origin. Since many patients have typical posterior overjet the use of Class II-Class III and anterior crisscross elastics...

Almodovar, A.; Suarez, J.; Nicola, G.; Nuevo, M., 2001: Genetic introgression between wild and stocked brown trout in the Douro River basin, Spain. The genetic diversity of Spanish brown trout is currently threatened by stocking with exogenous brown trout from Central and Northern Europe. In the Douro River basin 25% of the analysed populations in the present study showed introgression by gen...

Pecora M.J.; Roubin G.S.; Cobbs B.W.Jr; Ellis S.G.; Weintraub W.S.; King S.B.IIi, 1988: Presentation and late outcome of myocardial infarction in the absence of angiographically significant coronary artery disease. To determine the natural history of myocardial infarction (MI) in the absence of angiographically significant (no lesion .gtoreq. 50% diameter stenosis) fixed coronary artery disease (CAD), clinical and angiographic data and late outcome were stud...

Mennes, M., 2016: Commentary: Leveraging discovery science to advance child and adolescent psychiatric research--a commentary on Zhao and Castellanos 2016. 'Big Data' and 'Population Imaging' are becoming integral parts of inspiring research aimed at delineating the biological underpinnings of psychiatric disorders. The scientific strategies currently associated with big data and...

Anthony, H.M., 1989: Reactive changes in the blood of smokers and the development of arterial diseases and COPD, a review: evidence of associations between changes and subsequent disease with implications for the evaluation of harmful effects of cigarettes, and for susceptibility to the chronic effects of inhaled pollutants. The review examines the effects of smoking on blood parameters, concentrating on those seen as responses or reactions to the insult of smoking, and finds evidence that smoking causes chronic leucocytosis, macrocytosis and raised haematocrit, raise...

Maik, H., 1964: Photoabsorptiometric measurement of the density of bull semen Calibration of the photocolorimeter KF 3. The photocolorimeter KF 3 permits objective estimation of the density of bull semen with a high degree of accuracy, the av. total error in the estimate of sperm content per ml. semen being +or- 0.028x109. A correlation of 0.998 was found between p...

Henderson, C.F.; Brodsky, R.A.; Jones, R.J.; Levine, S.M., 2012: High-dose cyclophosphamide without stem cell rescue for the treatment of refractory Behcet's disease. Behcet's disease (BD) is a systemic inflammatory disorder characterized by recurrent mucocutaneous ulcerations, ocular inflammation, and numerous severe systemic inflammatory manifestations. While most patients respond to standard immunosuppr...

Y.Mei, T.L., 1997: Milk powder container. Official Gazette of the United States Patent & Trademark Office Patents 1202(3): 1920, Sept 16

Joy, M.; Revilla, R.; Gracia, S.; Delfa, R., 2006: Lamb growth and milk production and quality has been compared between Churra-Tensina ewes and their male lambs reared under two different managements. Ewes and lambs under the extensive (EXT) farming system were continuously stocked in the meadow together from birth to slaughter without any kind of supplement. Lambs under the intensive (INT) farming system always remained indoors, and ewes graze...

Vesely, F.J., 2014: Nematic-smectic transition of parallel hard spheroellipsoids. Spheroellipsoids are truncated ellipsoids with spherical end caps. If gradients are assumed to change smoothly at the junction of body and cap, the truncation height z0 determines the geometry uniquely. The resulting model particle has only two sh...