+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A serum factor in chronic hypocomplementemic hephritis distinct from immunoglobulins and activating the alternate pathway of complement

, : A serum factor in chronic hypocomplementemic hephritis distinct from immunoglobulins and activating the alternate pathway of complement. Journal of Experimental Medicine 139(5): 1249-1261

Nephritic factor (C3NeF) has been isolated from plasma of patients with hypocomplementemic chronic glomerulonephritis (HCG) by ion exchange and molecular sieve chromatography. This material was further treated with solidified anti-Ig antiserum. The purified material failed to react with antiserum to human IgG, IgG3, Fab, Fc, and kappa and lambda chains, but retained full C3NeF activity. The nonidentity of C3NeF with IgG was further demonstrated by Ouchterlony analysis using anti-IgG and anti-C3NeF. Isolated C3NeF was found to be a protein with a sedimentation coefficient of 7S and a mol wt of 150,000 daltons, which on microzone electrophoresis and gel electrophoresis at pH 8.6 behaved as a gamma-globulin. C3NeF is not a C1q precipitin and does not activate the classical complement pathway. Unlike cobra venom factor, it failed to enter into a complex with C3 proactivator (C3PA) when incubated with normal human serum (NHS) and then subjected to sucrose density gradient ultracentrifugation. The action of isolated C3NeF on C3 requires C3PA, C3PA convertase (C3PAse), and properdin (P). Similarly, C3PA conversion by C3NeF requires P, C3PAse, and C3. Total hemolytic activity was lost by incubation of 64 microg of C3NeF/1 ml NHS at 37 degrees C for 30 min. Both C3a and C5a anaphylatoxin could be generated by C3NeF in serum previously depleted of anaphylatoxin inactivator. Anti-C3NeF was found to detect an antigen in all NHS tested. Treatment of NHS with solidified anti-C3NeF caused impairment of the alternate complement pathway. It failed to sustain lysis of glutathione-treated human erythrocytes initiated by inulin. It is conceivable that the normal serum constituent which is removed by anti-C3NeF constitutes the inactive precursor of C3NeF, and a heretofore unrecognized component of the alternate pathway.


PMID: 4207623

Other references

Wyrick, W., 1968: The development of a test of motor creativity. Research Quarterly 39(3): 756-765

Heidari, M.; Sattarahmady, N.; Javadpour, S.; Azarpira, N.; Heli, H.; Mehdizadeh, A.; Rajaei, A.; Zare, T., 2016: Effect of Magnetic Fluid Hyperthermia on Implanted Melanoma in Mouse Models. Nowadays, magnetic nanoparticles (MNPs) have received much attention because of their enormous potentials in many fields such as magnetic fluid hyperthermia (MFH). The goal of hyperthermia is to increase the temperature of malignant cells to destr...

Chebotareva, L.I., 1956: Experience in treating seminoma of the testes and its metastases with sarcolysin. Voprosy Onkologii 2(3): 323-328

Sneh A., 1979: The fluviatile loess of the Bsor Basin. Pages 40 1979

Morisaki, T.; Ohba, K.; Yoshida, A.; Mizuta, Y.; Nakao, K., 2010: A case of hepatic portal venous gas caused by transient type ischemic enteritis. We report a case of hepatic portal venous gas (HPVG) caused by transient type ischemic enteritis. The patient was a 65-year-old woman, previously given a diagnosis of hypertension and diabetes mellitus. She was admitted to our hospital because of...

Wannlund, J.; DeLuca, M., 1983: Bioluminescent immunoassays. The procedures described here have been developed for only a few antigens at present. It seems very likely that they can be extended to any other antigen of choice. For sensitivities in the picomole range, the luciferase-antigen procedure is satis...

Vignes, J-R.; D.S.ze, M.; Sesay, M.; Barat, M.; Guerin, J., 2003: Anterior sacral root stimulation with dorsal rhizotomy (Brindley technique). Patients with spinal cord lesions are exposed to vesico-sphincter dysfunctions which can aggravate the vital or functional prognosis. The pathophysiological characteristics of these neurogenic bladders explains the usefulness of surgical treatment...

Gintant, G., 2015: In Vitro Early Safety Pharmacology Screening: Perspectives Related to Cardiovascular Safety. In vitro screening for cardiovascular safety liabilities of novel drug candidates presents a challenge for the pharmaceutical industry. Such approaches rely on detecting pharmacologic effects on key components of complex integrated system early in...

Ogusky, J.; Tenner, A., 2010: Advocating for schools to provide effective HIV and sexuality education: a case study in how social service organizations working in coalition can (and should) affect sustained policy change. Advocates believed that to slow an expanding HIV/ AIDS epidemic in Washington, D.C., a local effort could ensure that HIV prevention was brought to scale. Schools were chosen as the focus and a new coalition advocated for the city government to pa...

Piechocki, R., 1964: Uber die Vogelverluste im strengen Winter 1962/63 und ihre Auswirkungen auf den Brutbestand 1963. Falke, 11: 10-15