geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A set of anti-crystallin monoclonal antibodies for detecting lens specificities: beta-crystallin as a specific marker for detecting lentoidogenesis in cultures of chicken lens epithelial cells


, : A set of anti-crystallin monoclonal antibodies for detecting lens specificities: beta-crystallin as a specific marker for detecting lentoidogenesis in cultures of chicken lens epithelial cells. Japanese Journal of Ophthalmology 37(4): 355-368

Seven hybridoma lines which produced monoclonal antibodies against lens crystallins were established. They could detect alpha A-, alpha B-, gamma-, delta-, and several beta-crystallins of different species of animals with high avidities. Although immunohistological analysis of chicken and mouse lenses showed the typical distributions of each crystallin as have been reported so far, the ectopic expression of alpha B or delta-crystallin was observed in the brain or the kidney of chicken, respectively. Subsequently, crystallin production during the process of lentoidogenesis in cultures of chicken lens epithelial cells was examined with these antibodies. Western blot analysis revealed that beta-crystallins were not detected in cultured cells before lentoidogenesis, although all other crystallins were detected throughout the culture period. Immunostaining of cultures indicated clearly that expression of beta-crystallins was restricted to lentoid bodies. These data confirmed the lens fiber specificity of beta-crystallins as previously reported in the messenger RNA level. In addition, we found that small-size delta-crystallin (48 kDa) accumulated before the onset of lentoidogenesis. These results strongly suggest that the differentiated state of lens cells in vitro could be classified by examination of the expression pattern of crystallins. In addition, the anti-crystallin monoclonal antibodies produced in this study could be useful for detecting lens specificities.

US$29.90

PMID: 7832826


Other references

Okada, S., 1967: Investigations of the provenance character of Todomatsu (Abies sachalinensis) seedlings. 1. The variation and the difference of the cotyledon number among the provenances and mother trees. KEYWORDS: Abies sachalinensis provenance \ Abies sachalinensis seedlings \ Botany general \ tree breeding \ plant breeding \ Provenance and cotyledon number \ seeds \ weight and size relation \ cotyledon number \ Seedlings cotyledons

Brough, C., L.; Coventry, J., M.; Christie, W., W.; Kroon, J., T.M.; Brown, A., P.; Barsby, T., L.; Slabas, A., R., 1996: Towards the genetic engineering of triacyglycerols of defined fatty acid composition: Major changes in erucic acid content at the sn-2 position affected by the introduction of a 1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthes douglasii into oil seed rape. A cDNA encoding a 1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthes douglasii was introduced into oil seed rape (Brassica napus) under the control of a napin promoter. Seed triacylglycerols from transgenic plants were analysed by reve...

Wahl, H.Günther.; Graf, S.; Renz, H.; Fassbinder, W., 2004: Elimination of the cardiac natriuretic peptides B-type natriuretic peptide (BNP) and N-terminal proBNP by hemodialysis. Clinical Chemistry 50(6): 1071-1074

Ferguson, M.M.; MacDonald, D.G., 1978: Persistent sialadenitis in an accessory parotid gland. The patient who was followed had a persistent painful condition of the side of her face. Following a total parotidectomy, the symptoms presevered and this was ultimately diagnosed as being due to sialadenitis in the accessory parotide gland as wel...

Davino A., 1976: Geophysical surveys in the Serra de Aracoiaba and vicinity, Sao Paulo. Anais da Academia Brasileira de Ciencias 48(3): 531-545

Viscarret P.; Guerrero O.; Andara A., 2005: Igneous and metamorphic petrography atlas for the Sierra Nevada de Merida, Venezuela. Geos (Caracas) 38: 128

Chakrabarti S.; Maity S.P., 1980: New genus new species and new records of cerataphidine aphids homoptera aphididae from northwestern himalaya india. Aleurodaphis antennata sp. nov. and INDOREGMA bambusae gen. et sp. nov. infesting Bambusa spp. are described from Garhwal Himalaya of northwestern India. The relationships of the new genus and new species with the known ones were also discussed. C...

Ehinger, B.; Gustafsson-Sporrong, B., 1966: Adrenergic and cholinergic axons in the mouse iris. Comparison of the number of cholinergic and adrenergic fibers in the Schwann cells of the mouse iris shows that they are largely concomitant.

Genelhu, V.A.; Francischetti, E.A.; Duarte, S.F.P.; Celoria, B.M.J.; Oliveira, R.C.; Cabello, P.H.; Pimentel, M.M.G., 2010: Beta3-adrenergic receptor polymorphism is related to cardiometabolic risk factors in obese Brazilian subjects. We determined whether ADRbeta3 polymorphism is associated with obesity-related traits in 140 obese patients. Molecular analysis was performed by PCR and RFLP. Individuals carrying the Arg64 allele had a lower waist-to-hip ratio, higher adiponectin...

Kitano H.; Nogaki M.; Nogaki M.; Ozeki T.; Masumori S.; Kimura H.; Fuzino K.; Nogaki M., 1984: Detailed screening for colorectal cancer by sequential use of colonoscopy and barium enema. Colorectal cancers especially early cancers were analyzed. These were detected during a 5-yr period from 1978 when colonoscopy was begun and evaluated the results of screening. Colorectal cancers (404) including 121 early cancers were detected. A...