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A set of inhibitors for discrimination between the basic isozymes of glutathione transferase in rat liver

, : A set of inhibitors for discrimination between the basic isozymes of glutathione transferase in rat liver. Biochemical and Biophysical Research Communications 114(2): 829-834

A set of inhibitors including hematin, bromosulfophthalein, and triethyltin bromide was used for discrimination and identification of the major basic isozymes of glutathione transferase in rat liver cytosol. Six enzymes are formed as binary combinations of 4 protein subunits: A, B, C, and L. Discrimination between the transferases can be based on the differences of the subunits in susceptibility to the inhibitors. The identification of transferase subunits is further supported by the combined use of specific substrates and inhibitors.


PMID: 6882456

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