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A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322

, : A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322. Gene 16(1-3): 21-26

Seven oligonucleotide primers complementary to the plasmid vector pBR322 at positions adjacent to five of the unique restriction endonuclease cleavage sites (EcoRI, HindIII, BamHI, SalI and PstI) have been chemically synthesized. The polarity of the primers is such that any DNA inserted at one or a combination of two of the above restriction sites may be sequenced by the chain termination method using one of the synthetic DNA primers. One of the primers for sequencing inserts at the PstI site of pBR322 is also complementary to the M13 phage vector designated bla6. This set of universal primers is useful for rapid sequence determination of DNA cloned into pBR322 or M13bla6.


PMID: 6282692

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Jadczyk, P., 2001: Mutagenicity of organic pollutants adsorbed on airborne particles in the center of Wrocław. With Ames assay there was examined mutagenicity of airborne particles that was sampled in summer and winter in centre of Wrocław; there were also examined fractions of aliphatic hydrocarbons, aromatic hydrocarbons and polar compounds obtained fro...

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Labuda, D.; Grosjean, H., 1981: Aminoacids and the anticodon: anticodon interaction: a test of the stereochemical hypothesis?. Biochimie 63(1): 77-81

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