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A simple, sensitive protein-binding assay for guanosine 3':5'-monophosphate

, : A simple, sensitive protein-binding assay for guanosine 3':5'-monophosphate. Proceedings of the National Academy of Sciences of the United States of America 68(4): 736-739

A protein-binding assay for guanosine 3':5'-monophosphate, which employs an enzyme preparation from lobster tail muscle, is described. The binding constant for cyclic GMP was 2-10 nM for four different enzyme preparations. The method can be set up in several days and permits the detection of as little as 0.5-1 pmol of cyclic GMP. In some instances, it is possible to assay the nucleotide in urine or deproteinized extracts of tissue without purification. For other samples, a simple method for separating and purifying cyclic AMP and cyclic GMP is described.


PMID: 4323786

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