+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

ADP-ribosylation in inner membrane of rat liver mitochondria

, : ADP-ribosylation in inner membrane of rat liver mitochondria. Proceedings of the National Academy of Sciences of the United States of America 80(11): 3188-3192

NAD+ glycohydrolase activity is found at high levels in submitochondrial particles. It leads to the reaction products ADP-ribose, nicotinamide, and small amounts of 5'-AMP. Furthermore, submitochondrial particles catalyze the exchange reaction: [adenosine-14C]ADP-ribose + NAD+ in equilibrium [adenosine-14C]-NAD+ + ADP-ribose. When submitochondrial particles are incubated with NAD+, mono(ADP-ribosyl)ation of protein molecules migrating with an apparent molecular weight of 30,000 in sodium dodecyl sulfate/polyacrylamide gel electrophoresis is demonstrable. Inhibitor studies suggest attachment of ADP-ribose to arginine residues. ADP-ribose bound to submitochondrial particles is rapidly turning over. The release of ADP-ribose from the protein is probably enzyme catalyzed. The rapid turnover, the specificity of the modification, and the inhibition of ADP-ribosylation by ATP and nicotinamide suggest a regulatory role of mono(ADP-ribosyl)ation of a protein in the inner mitochondrial membrane.


PMID: 6574480

Other references

Leung C.K.H.; Shiu R.P.C., 1981: Required presence of estrogen and pituitary factors for the growth of human breast cancer cells in athymic nude mice. Estrogen, prolactin and other pituitary factors are implicated in the etiology of human breast cancer. The effects of estrogen and factors from GH3 rat pituitary tumor cells on the growth of T-47D human breast cancer cells were tested in athymic n...

Sutton, D.D.; Starr, M.P., 1960: Intermediary metabolism of carbohydrate by Erwinia amylovora. Sonic extracts of E. amylovora were shown to contain the enzymes necessary for cyclic operation of the hexose monophosphate shunt in the aerobic metabolism of glucose. The enzymes of the Embden-Meyerhof pathway of glycolysis were demonstrated in p...

Grshibowski, S., 1987: The creation of the standardized system of computerized information supply in the field covered by the State Committee for the Agoindustrial Sector of the USSR (Gosagroprom). The field covered by Gosagroprom covers some 50 000 farms, 7800 enterprises in the food industry, 7400 building and construction enterprises and 19 600 supply industry and service enterprises in the USSR. At present there are some 500 computing ce...

Theilgaard Monch, K.; Raaschou Jensen, K.; Heilmann, C.; Andersen, H.; Bock, J.; Russel, C.A.; Vindelov, L.; Jacobsen, N.; Dickmeiss, E., 1999: A comparative study of CD34+ cells, CD34+ subsets, colony forming cells and cobblestone area forming cells in cord blood and bone marrow allografts. Cord blood (CB) has become an alternative source of hematopoietic progenitor cells (HPCs) for allogeneic transplantation. We have developed a new efficient protocol for CB collection. Using this method an average of 17.7 x 10(8) [range (6.8-29.6)...

De-Olmos, J.S., 1977: An improved horseradish peroxidase method for the study of central nervous connections. The HRP [horseradish peroxidase] tract-tracing method was modified by the introduction of cryoprotective agents, allowing the enzymatic reaction to take place at sub-zero temperatures. By using cold temperatures the enzymatic reaction could be bet...

Kerr, Richard A., 2010: Arctic Armageddon needs more science, less hype. Science 329.5992

Zytkiewicz, A.; Trebicka-Kwiatkowska, B.; Pietroń, K.; Woźniak, F., 1965: 2 cases of hemimelia in newborn infants. Polski Tygodnik Lekarski 20(16): 575-576

Green, M., 1949: Coliform bacteria in shrimp. Food Research 14(5): 395-400

Moore, M.C.; Cherrington, A.D., 1996: The nerves, the liver, and the route of feeding: an integrated response to nutrient delivery. Nutrition 12(4): 282-284

During, R.; Rudolf, K.; Rüting, M., 1987: Comparative coagulation studies in induced therapeutic abortions with prostaglandin. 30 healthy primigravidae aged 16-19 were treated (10 each) with PGF2 alpha, 15-Methyl-PGF2 alpha or Sulproston. Bleeding time, recalcification time, platelet count, fibrin, platelet adhesiveness, partial thromboplastin time and thromboplastin time...