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Addressed enzymatic fragmentation of RNA molecules


, : Addressed enzymatic fragmentation of RNA molecules. Molekuliarnaia Biologiia 14(1): 200-211

RNase H has been used for selective cleavage of RNA of MS2 and R17 bacteriophages and 16S RNA from E. coli ribosomes in the region of formation of heteroduplex composed of RNA and an oligodeoxyribonucleotide complementary to a certain part of it. The oligonucleotides used--d(C-T-C-A-T-G-T-T-), d(C-C-A-T-C-T-T-T-T) and d(T-T-T-C-C-A-T-C-T-T-T-T)--were synthesized by chemical methods. The molecular weight of the fragments produced on cleavage of the RNA of MS2 and R17 were estimated with the use of gel electrophoresis under denaturating conditions. The dependence of the enzyme activity on Mg2+ and Na+ concentration and of RNA cleavage on the RNA: oligodeoxyribonucleotide ratio was investigated.

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PMID: 6262632


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Mallory, B.; Steers, W.D.; D.G.oat, W.C., 1989: Electrophysiological study of micturition reflexes in rats. Electrophysiological techniques were used to examine the asynchronous and evoked activity on postganglionic nerves to the urinary bladder in the urethananesthetized rat. Distension of the bladder (0.4-0.6 ml) evoked reflex contractions of the blad...

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Sebek, S., 1978: Sacred Polyporus of Chignahuapan. Mykologicky sbornik 55(1-2): 38-39

Entine, J., 2002: Preordained peak performance: genetics and the barrier of athletics. This paper discusses the genetic explanations for the dominance of East Africans in distance running, and examines whether hard training can help runners defy the genetic odds. The issue of genetic engineering and gene therapy in athletics is also...

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Pedrazzi, F.; Bommartini, F.; Freddo, J.; Emanueli, A., 1981: A study of the possible interaction of indoprofen with hypoglycemic sulfonylureas in diabetic patients. Phenylbutazone was shown to impair tolbutamide metabolism with resulting potentiation of its hypoglycemic effect and increased danger of hypoglycemia. As indoprofen is widely used for treating rheumatic conditions, and in particular osteoarthritis...

Roach D., 1991: Fracture dynamics from fractal analysis of fracture surfaces. Program with Abstracts - Geological Association of Canada(Mineralogical Association of Canada(Canadian Geophysical Union, Joint Annual Meeting 16: 106