+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Affinity labeling of residues within Hv2 of guinea pig anti-azobenzenearsonate antibodies of different isotypes and from different strains

, : Affinity labeling of residues within Hv2 of guinea pig anti-azobenzenearsonate antibodies of different isotypes and from different strains. Biochemistry 20(7): 1989-1996

Anti-p-azobenzenearsonate (ARS) antibodies of IgG1 and IgG2 isotypes produced in inbred strain 13 and strain 2 guinea pigs were affinity labeled with N-(bromoacetyl)-3-[(p-arsonophenyl)azo]-L-tyrosine (BAAT) or N-(bromoacetyl)-p-arsanilic acid (BAA). BAAT was shown to modify approximately 50% of the binding sites specifically and BAA approximately 30%. Both reagents preferentially modified residues in the heavy (H) chain to the extent that it contained over 80% of the affinity label associated with the native molecule. At least 80% of label borne by the variable domain of the H chain (VH) was found in the second hypervariable region (Hv2). BAAT labeled all anti-ARS antibodies exclusively at position N-59, which contains a lysyl residue. BAA labeled predominantly tyrosine at N-57 and, to a lesser extent, lysine-59 and tyrosine-50. Comparison of Hv2 sequences in anti-ARS and in antibodies reactive with other haptens has shown that tyrosine at N-50 and N-57 as well as lysine at N-59 is distinctive of antibodies with anti-ARS specificity, thus implying their involvement in antigen binding. The predominant sequence of Hv2 was identical in anti-ARS IgG1 and IgG2 molecules induced in either inbred guinea pig strain following either carrier priming or conventional immunization. Although limited variability does occur among the various populations of anti-ARS antibodies in certain residue positions in Hv2, no significant differences in the binding affinities or in the indexes of heterogeneity were seen among the various kinds of anti-ARS antibodies.


PMID: 6784761

Other references

Milleret C.; Gary Bobo E.; Buisseret P., 1985: Responses of visual cortical cells area 18 to extraocular muscle stretch or nerve stimulation in cats and normal or deprived kittens. Neuroscience Letters Supplement (22): S298

Sesterhenn, I.A.; Bauer, J.J.; Mostofi, F.K.; Mcleod, D.G.; Srivastava, S.; Moul, J.W., 1996: Apoptosis regulator proteins P53 and BCL-2 are independent prognostic biomarkers in surgically treated clinically localized prostate cancer patients. Pathology International 46(SUPPL 1): 11

Wang KaiJin;; Chen LieZhong; Y.X.aoPing, 2006: Chemical constituents and antifungal activity of essential oil from Solidago canadensis. The chemical constituents and contents of essential oil from Solidago canadensis were analysed using GC-MS. The antifungal activities of the compounds against six agricultural pathogenic fungi were also assayed. Fifty compounds, constituting ~91.0...

Galle E.L., 1988: Quinoa processing. Cereal Foods World 33(8): 677

Williams, R.Arthur.; Hagerty, B., 2004: Depression research: nursing's contributions. Research and Theory for Nursing Practice 18(2-3): 117-122

Hahn K.M.; Hastie S.B.; Sundberg R.J., 1992: Synthesis and evaluation of 2 diazo 3 3 3 trifluoropropanoyl derivatives of colchicine and podophyllotoxin as photoaffinity labels reactivity photochemistry and tubulin binding. Derivatives of the tubulin polymerization inhibitors colchicine and podophyllotoxin bearing the photoreactive 2-diazo-3,3,3-trifluoropropanoyl (DTFP) group were synthesized for evaluation as potential photoaffinity labels of the tubulin binding si...

Gupta R.K.; Gupta S., 1977: Effect of intra cerebro ventricular administration of strepto lysin o on electro cardiogram in rabbits. IRCS (International Research Communications System) Medical Science Library Compendium 5(9): 430

Sanders, H.D., 1991: Polypharmacy in the Elderly: Drug reactions and interaction in the aged. The elderly tolerate smaller doses of most drugs, largely because changes in body fat content, water content, and muscle mass often lead to higher blood levels than in a young person of the same weight. Moreover, diminished renal blood flow and cr...

Matsuno, K.; Miyakawa, K.; Ezaki, T.; Kotani, M., 1990: The liver lymphatics as a migratory pathway of macrophages from the sinusoids to the celiac lymph nodes in the rat. A migratory pathway of macrophages as well as lymphatic communications from the liver to the celiac lymph nodes were studied both macroscopically and histologically. The injection of gelatinized carbon into the porta hepatis revealed a new pathway...

Abelson R.S., 1979: Calculations of the melting temperature of iron using the Monte Carlo method. Eos, Transactions, American Geophysical Union: 6, Pages 951. 1979.