geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Amino acid sequence of human beta-factor XIIa


, : Amino acid sequence of human beta-factor XIIa. Journal of Biological Chemistry 258(18): 10924-10933

Human factor XII was activated by limited proteolysis with trypsin, and the resulting beta-factor XIIa (Mr = 30,000) was isolated by DEAE-Sephacel column chromatography. The complete amino acid sequence of beta-factor XIIa was then determined on peptides produced by enzymatic digestion with either trypsin, chymotrypsin, or Staphylococcus aureus V8 protease and by chemical cleavage at methionyl and tryptophyl bonds. beta-Factor XIIa is a glycoprotein composed of a heavy chain (243 amino acid residues) and a light chain (9 amino acid residues), and these two chains are held together by a disulfide bond. The carbohydrate is attached to asparagine residue 61 in the heavy chain. The amino acid sequence of the heavy chain shows a high degree of homology to the corresponding regions of other plasma serine proteases, such as plasmin, thrombin, factor IXa and factor Xa, as well as the pancreatic digestive enzymes. These results demonstrate that factor XII is the precursor of a typical serine protease that participates in the coagulation cascade.

US$29.90

PMID: 6604055


Other references

Nacif, M.Souto.; Barranhas, A.Dias., 2014: Stratification of cardiovascular risk by cardiac computerized tomography: shall we keep on ignoring?. Arquivos Brasileiros de Cardiologia 100(5): 483-483

Talo, S.; Rytökoski, U.; Hämäläinen, A.; Kallio, V., 1996: The biopsychosocial disease consequence model in rehabilitation: model development in the Finnish 'work hardening' programme for chronic pain. To compare the effectiveness of diverse rehabilitation programmes, comparable data about their effects on maintaining or improving the residual function of the rehabilitation patients should be gathered. Current rehabilitation theories and assessm...

Lin, C.H.; Yu, Y.W.; Chen, T.J.; Tsa, S.J.; Hong, C.J., 2001: Association analysis for dopamine D2 receptor Taq1 polymorphism with P300 event-related potential for normal young females. P300 has been demonstrated abnormal for a variety of neuropsychiatric disorders, and heritability has been proposed. We analyzed the event-related potentials for three DRD2 genotype groups in 134 normal young females. The results demonstrate that...

Zinndorf, J., 1928: Die Versteinerungen aus den Tertiar-Ablagerungen von Offenbach a. M. I Teil. Die Conohylien des Rupeltones (Septarientones). Bericht des Offenbacher Vereins fuer Naturkunde, 66-68: 65

Anonymous, 1998: Tainted waters. Scientists have recently found that pharmaceutical chemicals present in the environment come from human medications rather than hazardous waste spills or industrial accidents. The body may excrete as much as 90 percent of a drug in a biologically...

Krag, S.; Andersen, H.B.onden; Sorensen, T., 1999: Circadian intraocular pressure variation with beta-blockers. Purpose: To investigate circadian intraocular pressure variation in patients treated with topical beta-blockers as monotherapy. Method: Circadian IOP curves were measured on 25 patients (47 eyes) treated with topical beta-blockers. IOP was recorde...

Van Den Branden C.; Van Den Sande P.; Decleir W., 1980: Pigments in some developmental stages of aurelia aurita. Unfed polyps of A. aurita are white. Fed with Artemia salina nauplii they turn to pinkish because of the canthaxanthin present in the food. The deep brown-red color of strobila and ephyra is due to melanin.

Eloyan, A.; Crainiceanu, C.M.; Caffo, B.S., 2014: Likelihood-based population independent component analysis. Independent component analysis (ICA) is a widely used technique for blind source separation, used heavily in several scientific research areas including acoustics, electrophysiology, and functional neuroimaging. We propose a scalable two-stage ite...

Wocior, S., 1984: Further studies on sour cherry flower biology. Three-year studies were carried out with 2 cultivars. In the cv. Kerezer, the period of effective pollination (PEP) of individual flowers was 2-3 days but the PEP of the trees was 6-11 days. Physiological maturity of the flowers was generally one...

Anonymous, 1997: Ambulatory care criteria set: intervention for domestic violence. Number 20, November 1996. Committee on Quality Assessment. American College of Obstetricians and Gynecologists. International Journal of Gynaecology and Obstetrics: the Official Organ of the International Federation of Gynaecology and Obstetrics 57(1): 93-94