+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An alteration in glucose metabolism associated with a defect in ketone body metabolism

, : An alteration in glucose metabolism associated with a defect in ketone body metabolism. Proceedings of the National Academy of Sciences of the United States of America 70(1): 210-214

Skin fibroblasts (CC-69) cultured from a patient with a unique syndrome of ketoacidosis associated with coenzyme A transferase (EC deficiency showed an altered pattern of carbohydrate metabolism. These cells used glucose at a rate significantly less than controls (125 against 680 nmol/mg per hr). The oxidation of [6-(14)C]glucose to (14)CO(2) by these cells was also significantly diminished (12 against 350 pmol/mg per hr), but [2-(14)C]pyruvate and [1,4-(14)C]succinate oxidation by these cells did not differ from that by control cells. Measurements of glycolytic intermediates showed a reduction of several intermediates in the CC-69 cells that confirmed an inhibition of glycolysis between fructose-1,6-bisphosphate and pyruvate. The apparent inhibition in these cells could be reversed by an extended incubation of the cells in a buffered glucose solution. After 18 hr of incubation in 2.5 mM glucose, glucose uptake by the CC-69 cells increased 20-fold to 2560 nmol/mg per hr, whereas the rate for control cells remained constant at 640 +/- 90. Concomitant with this increase, [6-(14)C]glucose oxidation rose from 8 to 2261 pmol/mg per hr while controls remained constant at 428 +/- 175. This change was not due to new enzyme formation because incubation with puromycin had no effect on the increased use of glucose. Mixing experiments demonstrated no transfer of a permeable inhibitor or activating substances. In view of the deficiency of coenzyme A transferase in these cells, the data suggest an indirect regulatory role for this enzyme in peripheral tissue glycolysis.


PMID: 4509653

DOI: 10.2307/62300

Other references

Goto H.; Shimizu K., 1968: Isolation and characteristics of cyto pathogenic viral agents from a case of infectious diarrhea in cattle ecbo virus. Research Bulletin of Obihiro Zootechnical University Series 1 5(3): 357-364

Lee, C.Yeng.; Abizaid, A., 2014: The gut-brain-axis as a target to treat stress-induced obesity. Frontiers in Endocrinology 5: 117-117

Lemperle, G.; Nicolau, P.; Scheiermann, N., 2011: Is there any evidence for biofilms in dermal fillers?. Plastic and Reconstructive Surgery 128(2): 84e-85e

Ruth, A-Jane.; Kitching, A.Richard.; Semple, T.J.; Tipping, P.G.; Holdsworth, S.R., 2003: Intrinsic renal cell expression of CD40 directs Th1 effectors inducing experimental crescentic glomerulonephritis. Evidence suggests that human and experimental crescentic GN results from Th1-predominant immunity to glomerular antigens. CD40/CD154 signaling plays a key role in initiating Th1 responses and may direct Th1 effector responses. The role of CD40 in...

Goh, P.M.; Alexander, D.J., 1994: Laparoscopic Billroth II Gastrectomy. The first successful totally intra-abdominal laparoscopic Billroth II gastrectomy was performed on February 10, 1992, by our group in Singapore. The patient was a 76-year-old Chinese man with a 2-year history of gastric ulcer, which presented with...

Pérez, R.; Castellanos, M.; Rodríguez-Huete, A.; Mateu, M.G., 2011: Molecular determinants of self-association and rearrangement of a trimeric intermediate during the assembly of a parvovirus capsid. The minute virus of mice (MVM) provides a simple model for the dissection of the molecular determinants of the self-assembly, stability, and dynamics of a biological supramolecular complex. MVM assembly involves the trimerization of capsid subunit...

Lueger, R.J., 1983: The use of the MMPI-168 with delinquent adolescents. Compared the standard MMPI and the MMPI-168 scores of 90 male delinquent adolescents. Raw score and T-score correlations were generally high and within acceptable limits, which indicates that MMPI-168 scores are useful with delinquent adolescents....

Anonymous, 2003: Author Index: Volume 81, 2003. An author index to volume 81.

Ukolov, A.A.; Bykov, V.I., 1967: Overwintering of winter hard wheat with different sowing times. Hordeiforme 9, derived from Triticum turgidum x T. aestivum, had a higher winter survival when sown on 20 September than when sown earlier; the bread wheat Moscow 2453 had a better survival and a higher content of disaccharides after all sowing da...

Vinson Michael D.; Luttge Andreas, 2005: Multiple length-scale kinetics; an integrated study of calcite dissolution rates and strontium inhibition. Through an integrated experimental approach, utilizing both vertical scanning interferometry (VSI) and atomic force microscopy (AFM), we have been able to directly link calcite (104) dissolution rates over multiple spatial and temporal scales. By...