geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Antimitotic activity of dehydroretronecine, a pyrrolizidine alkaloid metabolite, and some analogous compounds, in a rat liver parenchymal cell line


, : Antimitotic activity of dehydroretronecine, a pyrrolizidine alkaloid metabolite, and some analogous compounds, in a rat liver parenchymal cell line. Chemico-Biological Interactions 30(3): 325-336

The actions of 13 pyrrolic alcohols with similar chemical properties have been tested on cultured liver cells. Two, dehydroretronecine and dehydrosupinidine, were putative metabolites of hepatotoxic pyrrolizidine alkaloids; the remainder were synthetic. All were either mono- or bi-functional alkylating agents. Groups of cells were exposed to the compounds and were later stimulated to divide by changing the medium, then fixed, stained, and the proportions of cells in mitosis counted and compared with those in similarly treated control cells. Eleven compounds partially or completely inhibited cell division at concentrations of 10(-4) M or less. Bifunctional compounds, including dehydroretronecine and 2,3-bis-hydroxymethyl-1-methylpyrrole, had the highest antimitotic activity coupled with lowest cytotoxicity. The least chemically reactive compound, 3-hydroxymethyl-1-methylpyrrole, was neither antimitotic nor cytotoxic, whereas the monofunctional alkylating agents with highest reactivity, such as 3-hydroxymethyl-1,2-dimethylpyrrole, were the most toxic to the cells. The mitotic block occurred at a post-synthetic stage of the cell cycle, and affected cells could grow to a giant size.

US$29.90

PMID: 7379211


Other references

von Herrath, M.G.; Holz, A.; Homann, D.; Oldstone, M.B., 1998: Role of viruses in type I diabetes. Viral infections frequently elicit strong cellular and humoral immune responses. This bears the inherent danger of co-activating autoreactive lymphocytes, either through bystander activation by cytokines or through direct sharing of conformational...

Huhn, M.; Barth, S.; Matthey, B.; Klimka, A.; Diehl, V.; Engert, A., 1998: Highly efficient periplasmic expression of functional Pseudomonas exotoxin-based immunotoxins. Annals of Hematology 77(SUPPL 2): S84

Meinertzhagen, R., 1933: New birds from Lebanon and Syria. Troglodytes troglodytes syriacus (p.20), Lebanon; Francolinus francolinus billypayni (p.21), Syria.

Popova, A., 1929: Zur Biologie von Ephydra obscuripes Beck, aus den Gewassern des Eltonsees. Russische Hydrobiologische Zeitschrift Saratov, 8: 140-141

Stuart, A.E.; Hudspeth, A.J.; Hall, Z.W., 1974: Vital staining of specific monoamine-containing cells in the leech nervous system. Cell and Tissue Research 153(1): 55-61

Prikryl, K., 1970: Effect of the time of application of additional nitrogen on the yield of spring barley. Response to an additional 25 kg/ha N (as KNO3) was best when the application was made early in the season.

Wong, C.; Wong, S.; Tang, H.; Minocha, R.; Singh, R.; Grills, N., 2017: Use of skin-lightening products among outpatient attendees in a North Indian Hospital. In India, fair skin is a highly valued physical attribute. Skin lightener products comprise 60% of the dermatological market. This cross-sectional study was conducted in the general medical outpatient clinics of Champa Christian Hospital to elucid...

Zhang, L., 2012: Analysis of cortical lateralization during mental task of geometric figure rotation. Mean Kolmogorov entropy within one second is introduced and is used to analyze and localize the cortical lateralization based on spontaneous EEG and nonlinear dynamics. The results of analysis indicate: The conclusion drawn form the use of this me...

Niedowicz, D.M.; Nelson, P.T.; Murphy, M.Paul., 2012: Alzheimer's disease: pathological mechanisms and recent insights. Amyloidopathies cause neurodegeneration in a substantial portion of the elderly population. Improvements in long term health care have made elderly individuals a large and growing demographic group, marking these diseases as a major public health...

Aichinger, E., 1943: Plant and human com-munity, biological comparison. The author shows how plant associations, like human societies, are developed from pioneer settlements by gradual adaptive steps. In both cases, competition plays a decisive part; in both cases there are constructive, conservative, stabilizing, and...