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C5a-induced aortic contraction: effect of an antihistamine and inhibitors of arachidonate metabolism


, : C5a-induced aortic contraction: effect of an antihistamine and inhibitors of arachidonate metabolism. Journal of Pharmacology and Experimental Therapeutics 220(1): 102-107

Cleavage of the complement protein C5 by activation of the complement system yields a low molecular weight fragment, C5a, historically referred to as anaphylatoxin. Among the biological activities of C5a is the ability to contrast isolated smooth muscle. This report is concerned with initial studies identifying mediators responsible for C5a-induced contraction of isolated guinea-pig aortic rings. C5a, purified from yeast-activated guinea-pig serum, caused a concentration-related contraction of the isolated guinea-pig aorta. The H1 antagonist diphenhydramine partially inhibited the response of the aorta to C5a, indicating that released histamine was, at least in part, responsible for the contraction. 5,8,11,14-Eicosatetraynoate, an inhibitor of both the lipoxygenase and cyclooxygenase pathway of arachidonate metabolism, inhibited the C5a-induced aortic contraction to an even greater extent than diphenhydramine, suggesting that products of arachidonate metabolism also play a role in the contraction. The cyclooxygenase inhibitors aspirin and indomethacin did not inhibit the C5a-induced aortic contraction, yet a combination of aspirin and diphenhydramine or indomethacin and diphenhydramine completely inhibited the aortic response to C5a. The presence of aspirin did not affect the ability of diphenhydramine to inhibit the response of the aorta to exogenous histamine, thus suggesting that cyclooxygenase products were not affecting the reactivity of histamine at the target smooth muscle. These studies indicate that both histamine and cyclooxygenase products are mediators responsible for C5a-induced aortic contraction.

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PMID: 6118427


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Croes, E.A.; Alizadeh, B.Z.; Bertoli-Avella, A.M.; Rademaker, T.; Vergeer-Drop, J.; Dermaut, B.; Houwing-Duistermaat, J.J.; Wientjens, D.P.W.M.; Hofman, A.; Van Broeckhoven, C.; van Duijn, C.M., 2004: Polymorphisms in the prion protein gene and in the doppel gene increase susceptibility for Creutzfeldt-Jakob disease. The prion protein gene (PRNP) plays a central role in the origin of Creutzfeldt - Jakob disease (CJD), but there is growing interest in other polymorphisms that may be involved in CJD. Polymorphisms upstream of PRNP that may modulate the prion pro...

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Lawrason, F.D.; Cronkite, E.P., 1949: Incidental finding of megaloblastie-like cells in bone marrow of one of two swine with macrocytie anemia and aehlorhydria. haematological observations have been made on two swine for approximately seventeen months after exposure to atom bomb ionizing radiation at Bikini. Macrocytic anaemia and aehlorhydria were found in both animals and a hyperplastic, "megalobla...

Ruiter, B.; Rozemuller, E.H.; van Dijk, A.J.; Garssen, J.; Bruijnzeel-Koomen, C.A.F.M.; Tilanus, M.G.; Knol, E.F.; van Hoffen, E., 2007: Role of human leucocyte antigen DQ in the presentation of T cell epitopes in the major cow's milk allergen alphas1-casein. Background: Little is known about the association between human leucocyte antigen (HLA) and cow's milk allergy (CMA). The aim of the present study was to determine the HLA restriction of T cell clones (TCCs) specific to alpha s1-casein, the...

Wilson, S.E.; Clower, C.G.; Ota, K.Y.; Radauskas, B., 1971: Evaluating mental health associates. Hospital & Community Psychiatry 22(12): 371-372

Fitts, D.A.; Abel, P.L.; Lane, J.R.; Olavarria, J.F., 1994: Horseradish peroxidase leakage through the blood-brain barrier after an electrolytic lesion of the subfornical organ. Society for Neuroscience Abstracts 20(1-2): 820

Wotoweic, Peter, 1984: A peace corp beekeeper in Guatemala. Gleanings in Bee Culture 112: 1

J.L.Simonson, P.Steele, 1981: Cheliped asymmetry in the stone crab menippe mercenaria with notes on claw reversal and regeneration. Stone crabs (M. mercenaria) were reared in the laboratory to determine natural handedness. Claws of feral juvenile crabs were autotomized to test for reversal and to determine stridulatory pattern sequences. All crabs < 4-mm carapace width were...

Pelosi, L.A.; Voss, S.; Liu, M.; Gao, M.; Lemm, J.A., 2013: Effect on hepatitis C virus replication of combinations of direct-acting antivirals, including NS5A inhibitor daclatasvir. Three hepatitis C virus (HCV) inhibitors, asunaprevir (ASV; BMS-650032), daclatasvir (DCV; BMS-790052), and BMS-791325, each targeting a different nonstructural protein of the virus (NS3, NS5A, and NS5B, respectively), have independently demonstra...

d' Almeida, J.M.; Oliveira, V.C. de, 2002: Artificial diets for rearing Chrysomya (C. megacephala, C. albiceps e C. putoria) (Diptera: Calliphoridae) in the laboratory. A study was conducted to evaluate the post-embryonic development of species of Chrysoma (C. megacephala, C. albiceps and C. putoria) on 3 artificial diets. The diets were composed of the following: diet 1, whole dried milk+yeast extract; diet 2, s...