+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Catabolic pathways for glucose, glycerol and 6-phosphogluconate in Mycobacterium leprae grown in armadillo tissues

, : Catabolic pathways for glucose, glycerol and 6-phosphogluconate in Mycobacterium leprae grown in armadillo tissues. Journal of General Microbiology 129(5): 1481-1495

With radioisotopes, it was shown that suspensions of Mycobacterium leprae oxidized glycerol, 6-phosphogluconate, glucose, glucose 6-phosphate, and, at a low rate, gluconate, to CO2. The incubation period in these experiments was usually 20 h, but after 140 h up to five times more glucose and gluconate had been converted to CO2. Studies with differentially labelled glucose indicated that glycolysis and the hexose monophosphate pathway were used for glucose dissimilation. Key enzymes of glycolysis, the hexose monophosphate pathway and glycerol catabolism were detected in cell-free extracts from purified M. Leprae, but phosphoketolase, Entner-Doudoroff pathway activity and gluconate kinase were absent. All these enzymes were present also in host-tissue, but biochemical evidence is presented which indicates that all enzymes detected in extracts from M. leprae were authentic bacterial enzymes. Additionally, they could all be detected in extracts of M. leprae prepared by treatment with NaOH in which host enzymes adsorbed to M. leprae are inactivated.


PMID: 6352857

DOI: 10.1099/00221287-129-5-1481

Other references

Zenner, H.P., 1981: Cytoskeletal and muscle-like elements in cochlear hair cells. Monospecific antibodies to actin and to tubulin were used as immunofluorescent probes to evaluate the distribution of microtubules and actin filaments in the organ of Corti in mouse and guinea pig. The results indicate that in cochlear receptor ce...

Monmayrant, A.; Chatel, Béatrice.; Girard, B., 2006: Quantum state measurement using coherent transients. We present the principle and experimental demonstration of time resolved quantum state holography. The quantum state of an excited state interacting with an ultrashort chirped laser pulse is measured during this interaction. This has been obtained...

Röhner, G.; Rautenburg, H.W.; Höpfner, B., 1983: Transient hypothyroidism in infants following administration roentgen contrast media. Rontgenpraxis; Zeitschrift für Radiologische Technik 36(9): 301-304

Piasarolo,L.; Rigitano,R.L.deO.; Guerreiro,M.C., 2008: Influence of the polarity of non-ionic pesticides on their sorption by a latosoil. The sorption of thirteen non-ionic pesticides, with varying n-octanol/water partition coefficients (Kow), by soil materials taken from the A horizon of an acriferric red latosol was investigated. The sorption isotherms of the pesticides were deter...

Portillo Rubio, M., 1983: Atylotus flavoguttatus Szilady 1915, Tabanus s str albifrons Szilady 1914 y Tabanus s str mateusi Travassos Dias 1980 Diptera, Tabanidae nuevas especies para la fauna iberica, europea y; espanola, respectivamente. Actas do Congresso Iberico de Entomologia 1.2: 579-585

Saito, S., 1974: Mydriasis test in primary angle-closure glaucoma. Nippon Ganka Gakkai Zasshi 78(11): 1179-1185

Bachmann, M., 1979: Adapted techniques for the collection, processing and marketing of milk in developing countries. I. Some reflections on the place of milk in diet of the people in developing countries. A comparison is made of the price of milk and the daily wages of skilled and unskilled workers in several African, Asian and Latin-American countries. Results of this survey showed that production costs of 1 kg milk vary from 3 to 111% of the av....

Volynskii, V.N., 1990: Manifestations of the scale factor in the bending of wood. A theoretical analysis was made of the scale factor (size-effect) in bending, and this was complemented by experiments on the effect of dimension on the modulus of elasticity in the bending of test-pieces of pine [Pinus sylvestris]. In a conventio...

Tan, A.; Tuckey, J.; Rice, M., 2001: Orchidectomy following failure of antiandrogen monotherapy in patients with metastatic prostate cancer. Objective: Patients with metastatic prostate cancer who are initially treated by oral antiandrogens and then have progressive disease may be offered surgical castration as a second-line treatment. Twenty-eight such patients were reviewed to determ...

Porsdal, V.; Boysen, G., 1999: Direct costs during the first year after intracerebral hemorrhage. Objectives: Knowledge of resource use and associated costs of treatment, care and rehabilitation at hospitals and in the health care and social service sectors is limited. This study presents data on the total resource use during the first year af...