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Comparative kinetics of phosphomannosyl receptor-mediated pinocytosis of fibroblast secretion acid hydrolases and glycopeptides prepared from them


, : Comparative kinetics of phosphomannosyl receptor-mediated pinocytosis of fibroblast secretion acid hydrolases and glycopeptides prepared from them. Journal of Cellular Biochemistry 22(2): 69-86

In a previous report we demonstrated that phosphorylated oligosaccharides isolated from acid hydrolases were subject to pinocytosis by phosphomannosyl receptors present on the cell surface of human fibroblasts [9]. However, limiting quantities of oligosaccharides precluded detailed comparison of the kinetics of pinocytosis of these phosphorylated oligosaccharides to those of the acid hydrolases from which they were derived. In this report we present studies comparing the kinetics of pinocytosis of acid hydrolases from NH4Cl-induced fibroblast secretions with those of concanavalin A-binding glycopeptides prepared from them by pronase digestion. The uptake of both secretion acid hydrolases and 125I-labeled glycopeptides was linear for at least 3 hr, saturable, inhibited competitively by mannose 6-phosphate, and destroyed by prior treatment of the ligand with alkaline phosphatase. The inhibition constants of excess unlabeled glycopeptide for the uptake of 125I-labeled glycopeptides (Ki of 1.5 X 10(-6) M) and for the uptake of secretion acid hydrolases (Ki of 2.2 X 10(-6) M) were remarkably similar. Furthermore, the Ki for mannose 6-phosphate inhibition of pinocytosis of glycopeptide uptake (3 X 10(-5) M) compares closely to that previously determined for the pinocytosis of intact "high-uptake" acid hydrolases (3-6 X 10(-5) M). "High-uptake" fractions of both ligands were prepared and quantified by affinity chromatography on immobilized phosphomannosyl receptors purified from bovine liver. Only 10% of the concanavalin A-binding glycopeptides bound to the immobilized phosphomannosyl receptors, while 80% of the acid hydrolases from which they were prepared bound and were eluted with 10 mM mannose 6-phosphate. However, the fraction of each type of ligand that binds to the immobilized phosphomannosyl receptors accounts for all the uptake activity of that ligand.

US$19.90

PMID: 6323494

DOI: 10.1002/jcb.240220202


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Guevara-Suarez, M.; Llaurado, M.; Pujol, I.; Mayayo, E.; Martin-Vicente, A.; Gené, J., 2017: Fungal Olecranon Bursitis in an Immunocompetent Patient by Knoxdaviesia dimorphospora sp. nov.: Case Report and Review. Bursitis is a common medical condition that can occur either with or without infection. We present a case of fungal olecranon bursitis in an immunocompetent individual caused by the new species Knoxdaviesia dimorphospora. It is a dematiaceous fila...

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Dunleavy, J.M., 1956: Soybean diseases in Iowa in 1955. A survey of soy-bean diseases in Iowa in 1955 revealed that 95 per cent. of the fields were infected by root rot (Fusarium sp.), 65 per cent. by pod and stem blight, 28 per cent. by bacterial blight, and 23 per cent. by brown stem rot : loc.

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Orvig S., 1972: Hydrologic cycle of Greenland and Antarctica. Pages 23-32 1972

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Macmahon, H.E.; Urista, M.S., 1964: The Spread Of An Ependymoma From The Brain To The Lungs. A case of an ependymal cell tumor of the brain with distal metastases into the lungs and regional lymph nodes is reported. Tumor cells were demonstrated invading the vascular sinuses of the dura. As in each of the other five cases of ependymal cel...

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Clench, W.J., 1939: Helix nemoralis L. at Warm Springs, Va. Nautilus Philadelphia, 523: 108

Jacobsen, N., 1980: The Cryptocoryne albida group of mainland Asia (Araceae). Liber gratulatorius in honorem H C D de Wit redactie J C Arends et al: 204