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Concanavalin-A binding to acetylcholine receptors: identification of two forms of receptor in denervated rat muscle


, : Concanavalin-A binding to acetylcholine receptors: identification of two forms of receptor in denervated rat muscle. Journal of Pharmacology and Experimental Therapeutics 218(1): 27-33

The interaction of the nicotinic acetylcholine receptor from denervated rat muscle (AChR, solubilized in Triton X-100) and Concanavalin-A (Con-A) was studied by soluble Con-A competing with the binding of AChR to agarose immobilized Con-A, by immuno-precipitation of Con-A-AChR complexes and by inhibition of alpha-bungarotoxin binding to AChR. Results showed that Con-A bound to 85% of all AChR molecules at multiple cooperative binding sites. Con-A caused a partial inhibition of toxin binding to a maximum of 41% of receptors, but only when AChR was first incubated with Con-A before toxin labeling and not when the order was reversed. The blockade of toxin labeling was reversed when the Con-A-AChR complexes were treated with mannose (0.75 M). Receptor-Con-A complexes in which toxin binding was not inhibited showed saturation binding of toxin with a decreased apparent affinity. A sub-population of AChR prepared by affinity purification on Lens Culinaris lectin-agarose columns was 100% inhibited from toxin binding when preincubated with Con-A. We conclude that receptors from denervated rat muscle which bind Con-A (85% of the total AChR) contain two major subpopulations of AChR in the approximate proportions 6:4 that can be distinguished by interactions with lectins having specificity for mannose.

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PMID: 7241383


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Takahashi, M.; Tanonaka, K.; Yoshida, H.; Koshimizu, M.; Oikawa, R.; Daicho, T.; Takeo, S., 2005: Effects of angiotensin I-converting enzyme inhibitor and angiotensin II type 1 receptor blocker on the right ventricular sarcoglycans and dystrophin after left coronary artery ligation. We examined the effects of trandolapril and candesartan on changes in the levels of sarcoglycans and dystrophin in the fight ventricle of rats with the left coronary artery ligation. Hemodynamic and morphological alterations suggested the developm...

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Singh, J.P.; Srivastava, O.P., 1994: Soil testing and plant analysis for sulfur nutrition of potato in orthent. Communications in Soil Science & Plant Analysis 25(7-8): 704

Boehnel, J.; Stacher, A., 1964: Experiences With A Prednisolone Nicotinic Acid Ester In Hematologic Diseases. Therapeutische Umschau. Revue Therapeutique 21: 224-229

Kayser, Jürgen.; Tenke, C.E.; Kroppmann, C.J.; Fekri, S.; Alschuler, D.M.; Gates, N.A.; Gil, R.; Harkavy-Friedman, J.M.; Jarskog, L.F.; Bruder, G.E., 2010: Current source density (CSD) old/new effects during recognition memory for words and faces in schizophrenia and in healthy adults. We previously reported a preserved 'old-new effect' (enhanced parietal positivity 300-800 ms following correctly-recognized repeated words) in schizophrenia over mid-parietal sites using 31-channel nose-referenced event-related potential...

Roalstad, M.S., 1989: Physiologic testing of the ultraendurance triathlete. With the ever growing popularity of the sport of triathlon, numerous studies have been done in an attempt to characterize the triathlete and to identify physiologic variables important to success in triathlon racing. Studies of the ultraendurance...

Glasa, J.; Holomán, J.; Stancek, D.; Horváth, A.; Volmut, J.; Kupcová, V.; Páleníková, O.; Brixová, E., 1987: The asymptomatic carrier state of viral hepatitis B. Vnitrni Lekarstvi 33(9): 748-754

Graham D.Y., 1984: The role of cell wall defective pseudomonas maltophilia in crohns disease dna dna hybridization in situ. Gastroenterologypart 2: 1305