+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Culture conditions induce the appearance of immortalized C3H mouse cell lines

, : Culture conditions induce the appearance of immortalized C3H mouse cell lines. In Vitro 20(8): 597-601

Mouse fibroblasts were cultured by three different procedures: (a) changing the 0.2 ml/cm2 of growth medium every 2nd d and seeding 1 X 10(5) cells/cm2 after confluency; (b) changing the 0.4 ml/cm2 of growth medium only at subculture performed at confluency by a 1:2 split and keeping the bottles incubated on a rocking platform; (c) the same as Method b but keeping the bottles stationary throughout culture. By Method a no lines were immortalized over 36 experiments whereas Method b gave 1/4 immortalized lines and Method c gave 10:12 immortalized lines. Cells always went into crisis at the 9th to 11th doubling. Immortalized lines had a tetraploid DNA content.


PMID: 6500597

Other references

Massee, A.M., 1942: Aphis transmission of strawberry crinkle in Great Britain. Mild symptoms of Crinkle were induced in healthy plants of the common woodland strawberry (Fragaria vesca) by transfer and feeding of either adult or immature stages of apterous viviparous $ aphids (Capitophorus fragariae) previously fed on plants...

Jagtap, A.G.; Karkera, S.G., 2000: Potential of the aqueous extract of Terminalia chebula as an anticaries agent. The aqueous extract from Terminalia chebula was tested for its ability to inhibit the growth and some physiological functions of Streptococcus mutans. The extract strongly inhibited the growth, sucrose induced adherence and glucan induced aggregat...

Nakagawa, K.; Horiuchi, Y.; Mashimo, K., 1969: Responses of plasma growth hormone and corticosteroids to insulin and arginine with or without prior administration of dexamethasone. Journal of Clinical Endocrinology and Metabolism 29(1): 35-40

Simmons, R., 1991: Wading birds of the Sandwich Harbour wetlands, February, 1991. Preliminary estimates and alternative counting methods. Lanioturdus, 261: 4-15

Frattini, B., 1936: The presence of folliculin in the male organism. SPAYED rats were given daily injections of 500 I.U. of the oestrogenic substance from either (a) pregnancy urine, or (b) stallion urine. In both lots the uteri were greatly enlarged. Animals in lot (a) had pituitaries 3-4 times the volume of the g...

Paris, C.G.; Magasanik, B., 1981: Tryptophan metabolism in Klebsiella aerogenes: regulation of the utilization of aromatic amino acids as sources of nitrogen. Klebsiella aerogenes utilized aromatic amino acids as sole sources of nitrogen but not as sole sources of carbon. K. aerogenes abstracted the alpha-amino group of these compounds by transamination and excreted the arylpyruvate portions into the me...

O'neill R.V., 1972: Examples of ecological transfer matrices. US-IBP (International Biological Program) Analyses of Ecosystems Program Interbiome Abstracts 2(1): 24-25

Morton, H., 1985: The television advertising of foods to children: a South Australian study. A recent South Australian study assessed the type and frequency of food advertisements broadcast on 3 commercial television stations during 30 hours of programming. The food advertisements (as per cent of all advertisements) shown during children...

Elvira, G.; Moreno, B.; Valle, I.Del.; Garcia-Sanz, J.A.; Canillas, Mía.; Chinarro, E.; Jurado, Jé.R.; Silva, A., 2012: Targeting neural stem cells with titanium dioxide nanoparticles coupled to specific monoclonal antibodies. Aiming to characterize the use of biomaterials in cancer therapy, we took advantage of the n-type semiconductor properties, which upon irradiation excite their electrons into the conduction band to induce photoelectrochemical reactions generating...

Lendvai Karoly, 1969: Problems of geophysical terminology. Foldtani Kutatas 12(3-4): 76-77