+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Detection of anti-teichoic acid immunoglobulin G antibodies in experimental Staphylococcus epidermidis endocarditis

, : Detection of anti-teichoic acid immunoglobulin G antibodies in experimental Staphylococcus epidermidis endocarditis. Infection and Immunity 42(3): 1020-1026

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rabbit immunoglobulin G (IgG) antibodies to purified cell wall teichoic acids from the Staphylococcus aureus Lafferty strain and three strains of coagulase-negative staphylococci. Significant immunological cross-reactivity occurred only between the teichoic acid of S. aureus and one coagulase-negative preparation. The ELISA was used to determine the serum IgG response to Staphylococcus epidermidis in a rabbit model of aortic valve endocarditis. Blood samples were drawn before inoculation and then every 5 days until death or sacrifice at 32 to 35 days postinoculation. Valve vegetations were culture positive at autopsy in 16 (59%) of the 27 catheterized rabbits. Antibody titers in this culture-positive group and the culture-negative group began to rise as early as day 6. Although both groups demonstrated an antibody response, the culture-positive group attained a significantly higher titer on days 26 and 31. Antibodies also rose in a control group of rabbits without a heart catheter but which were inoculated with bacteria. Again, the antibody titer was significantly less than that for the culture-positive group. This ELISA may be useful for the diagnosis of coagulase-negative staphylococcal infections in humans.


PMID: 6642658

Other references

Yung, A.; Goodfield, M.D., 2003: An annular lesion on the back. Archives of Dermatology 139(9): 1209-1214

Miyamura, M.; Xi, L.; Ishida, K.; Schena, F.; Cerretelli, P., 1990: Effects of acute hypoxia on ventilatory response at the onset of submaximal exercise. In order to investigate the effects of acute hypoxia and accompanying hypocapnia on the ventilatory response at the onset of dynamic exercise, four healthy adult men performed 50W rectangular loads on a cycle-ergometer in normoxic (FIO2 = 0.21) an...

Parker, R.J.; Bhatti, M.A., 1982: Selection for feed efficiency in mice under AD libitum and restricted feeding terminated by fixed time or quantity of intake. Mass selection for feed conversion efficiency (FCE) in mice was practiced for six generations in two lines fed ad libitum (AL) and two lines restricted to 80% of ad libitum (R). In one line of each pair of selected lines and test period was termin...

Allison, C.P.; Tempelman, R.J.; Doumit, M.E., 2001: Analysis of postmortem tenderization in porcine Longissimus dorsi muscle. Journal of Dairy Science 84(Supplement 1): 20

Woodbury, D.M.; Karler, R., 1960: The role of carbon dioxide in the nervous system. Anesthesiology 21: 686-703

Strassmann, JE., 1982: Sex investment ratios in Polistes exclamans. Unknown

Van Blerkom, J.; Davis, P., 2006: High-polarized (Delta Psi m(HIGH)) mitochondria are spatially polarized in human oocytes and early embryos in stable subplasmalemmal domains: developmental significance and the concept of vanguard mitochondria. The spatial specificity and stability of subplasmalemmal domains of high-polarized mitochondria (Delta Psi m(HIGH)) in human oocytes and cleavage stage embryos were investigated in instances where changes in pericortical/subplasmalemmal organizati...

Guo, M.; Huang, J.; Hu, H.; Liu, W., 2012: Growth and repair potential of three species of bacteria in reclaimed wastewater after UV disinfection. The growth and repair potential of three typical microorganisms in reclaimed water after UV disinfection was investigated to assess the effects of photo-reactivation and dark repair of microorganisms, and the microbial safety of reclaimed water fo...

Mohseni, M.; Lopez, M.D., 2008: Images in emergency medicine. Uvular angioedema (Quincke's disease). Annals of Emergency Medicine 51(1): 8, 12-8, 12

Gonzalez, L.M.riano; Oria, J.; Margalida, A.; Sanchez, R.; Prada, L.; Caldera, J.; Aranda, A.o; Molina, J.I.nacio, 2006: Effective natal dispersal and age of maturity in the threatened Spanish imperial eagle Aquila adalberti: conservation implications. Capsule Dispersive capacity appears sufficient to establish natural re-occupation of the former range. Aims To document data on effective natal dispersal and age of maturity in the increasing, but fragmented, Spanish Imperial Eagle population. Met...