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Developmental alterations in guanine nucleotide regulation of the beta-adrenergic receptor-adenylate cyclase system of skeletal muscle

, : Developmental alterations in guanine nucleotide regulation of the beta-adrenergic receptor-adenylate cyclase system of skeletal muscle. Journal of Biological Chemistry 259(11): 7294-7299

The postnatal development of skeletal muscle is accompanied by an increased capacity for glycogenolysis and anaerobic glycolysis. In the present study, regulatory features of cAMP synthesis were examined in neonatal and adult rabbit sarcolemmal membranes. Adult sarcolemma exhibited a 3-, 6-, and 10-fold greater adenylate cyclase activity than neonate for basal, NaF, and isoproterenol plus GTP, respectively. The Km for activation by isoproterenol was 1.4 X 10(-8) M and 6 X 10(-8) M for GTP. The number of beta-receptors was similar (0.9-1.2 pmol/mg). 10 microM GTP shifted isoproterenol EC50 from 1 X 10(-8) M to 1 X 10(-7) M in adult; neonatal agonist affinity was unaffected by GTP. Cholera toxin stimulated adenylate cyclase activity 2-fold and catalyzed 32P ribosylation of a Mr = 42,000 peptide in adult sarcolemma; both activities were low or absent in neonate. Isoproterenol-stimulated GTPase activity was elevated 4-fold in adult compared to neonatal sarcolemma. Mn2+ ion-stimulated basal activity, an indicator of catalytic function of adenylate cyclase, was also elevated in adult. Together, these findings suggest that the development of catecholamine-sensitive cAMP synthesis in muscle is governed by the coordinate expression of the regulatory and catalytic proteins of adenylate cyclase, but not the beta-receptor.


PMID: 6327715

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Blond S.; Coche Dequeant B.; Castelain B.; Meynadier J.; Madelain M.; Clarisse J.; Demaille A.; Christiaens J L., 1990: Stereotactic radiosurgery method and indications in the treatment of cerebral arteriovenous malformations. Agressologie 31(5): 277-279

Altura, B.M., 1978: Magnesium withdrawal and rhythmic contractility of arterial vs venous smooth muscle differential effects of multivalent cations and edta. Acute changes in [Mg2+]o affect contractile activity of arterial, arteriolar and venous smooth muscle. Withdrawal of [Mg2+]o can induce rapid and potent contractions of arterial and venous smooth muscle. Addition of foreign divalent (i.e., Ni2+, C...

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Stahl, R.A.; Attallah, A.A., 1980: Sar1-Ile8-angiotensin II decreases urinary PGE2 and PGF2alpha excretion in rabbits on dietary sodium restriction. Urinary PGE2 and PFG 2 alpha excretion (UPGV) were determined prior, during and after 48 h treatment with angiotensin-II-blocker, Sar1-Ile8-angiotensin II (200 microgram/kg/3 h), in rabbits maintained on chronic low (LS) or high (HS) salt diets. U...

Imreh I., 1977: Migration of Sr, Ba, and Pb in Tertiary limestones. Studia Universitatis Babes-Bolyai Geographia

Perach, M.; Hizi, A., 1999: Catalytic features of the recombinant reverse transcriptase of bovine leukemia virus expressed in bacteria. A recombinant reverse transcriptase (RT) of bovine leukaemia virus (BLV)was expressed in bacteria such that the gene encoding the RT started at the 5' end next to the last codon of the mature viral protease; the amino terminus of the RT match...

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Davis, B.M.; Bernstein, R.; Kolterman, O.; Olefsky, J.M.; Reaven, G.M., 1979: Defect in glucose removal in nonketotic diabetic patients with fasting hyperglycemia. Experiments were performed to determine the ability of insulin to stimulate glucose uptake in normal subjects as compared with nonobese patients with fasting hyperglycemia at similar steady state plasma glucose and insulin concentrations. Patients...

Anonymous, 1978: Board of Regents Health Manpower Planning Project. The LSNA point of view. Pelican News 34(2): 10-11