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Effect of calcium phosphate and alumina C gamma gels on the mutagenicity and cytotoxicity of dimethylnitrosamine as studied in the CHO/HGPRT system


, : Effect of calcium phosphate and alumina C gamma gels on the mutagenicity and cytotoxicity of dimethylnitrosamine as studied in the CHO/HGPRT system. Mutation Research 84(1): 147-156

When CaCl2 was added in increasing concentrations to a rat liver metabolic activation system (S9) buffered with sodium phosphate, the mutagenic activity and cytotoxicity of dimethylnitrosamine (DMN) in the Chinese hamster ovary cell/hypoxanthine-guanine phosphoribosyl transferase (CHO/HGPRT) system were greatly increased. This effect was not observed with an S9 mix buffered with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES). The calcium phosphate gel precipitate of the S9 mix possessed approximately 1/3 of the total activity of the mix, while the supernatant had only slight activity. However, when the calcium phosphate gel precipitate of a solution of S9 salts (without S9 protein) was added to the supernatant, the remaining 2/3 of the activity was recovered. Commercially obtained calcium phosphate, tricalcium phosphate, and alumina C gamma gels could substitute for CaCl2 in the S9 mix, but diethylaminoethyl cellulose (DEAE cellulose) could not. Alumina C gamma gel can exert its effect in the absence of both CaCl2 and phosphate in the S9 mix. Increasing the time of contact between the S9 protein and the S9 salts increased the efficacy with which the S9 mix activated DMN; this is indicative of an adsorptive process by calcium phosphate gel.

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PMID: 7199116


Other references

Strohecker H.F., 1979: Endomychidae from the indo malayan region with descriptions of 13 new species coleoptera. New species of Endomychidae are described from Borneo and Southeast Asia. Described as new are Mycetina bakeri, M. rhodoptera, Chondria crenata, C. elegans, Danae borneensis, Tragoscelis angustus from Sabah [Borneo]; Danae hirsutipes from Sabah an...

Brown, B., 1980: Barred owl-human fisherman interaction. Kentucky Warbler, 563: 66-67

Jiao, L., 2010: Archaeology. Unprecedented excavation brings Maritime Silk Road to life. Science 328(5977): 424-425

Rabkin, S.W., 2016: Nonsustained monomorphic ventricular tachycardia following arousal from sleep after face trauma. International Journal of Cardiology 181: 3-4

Naumova, V.I.; Sitnikova, V.P.; Larenysheva, R.D.; Kunakova, N.F., 1984: Staged treatment and dispensary care of children with urinary diseases. Pediatriia: 3-6

Kuzyakin, A.P. ., 1964: On the systematics of the Soviet rodents. Unknown

Nemethy, Z.; Kovacs, K.J., 1994: Time course of induction of ACTH and activation of Fos-expression in endotoxic shock. Neuroendocrinology 60(SUPPL 1): 45

Mohan, R.R.; Pianotti, R.S., 1960: Studies on the mode of action of colistin sulfate on Pseudomonas aeruginosa ATCC 9027. Bact Proc 60: 99

Arai, K.Y.; Kishi, H.; Onodera, S.; Jin, W.; Watanabe, G.; Suzuki, A.K.; Takahashi, S.; Kamada, T.; Nishiyama, T.; Taya, K., 2005: Cyclic changes in messenger RNAs encoding inhibin/activin subunits in the ovary of the golden hamster (Mesocricetus auratus). To elucidate changing patterns of inhibin/activin subunit mRNAs in the ovary of the golden hamster (Mesocricetus auratus) during the oestrous cycle, inhibin/activin subunit cDNAs of this species were cloned and ribonuclease protection assay and in...

Rossi, D.; Epp, D.J., 1979: Estimating the demand for outdoor recreation: a review of literature. The literature reviewed was published between the late 1950s up to 1976, and tends to fall into three general categories. The first covers discussions of and variations in the so-called Hotelling-Clawson-Knetsch model, while the second deals with...