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Effects of natural sequence variation on recognition by monoclonal antibodies neutralize simian immunodeficiency virus infectivity


, : Effects of natural sequence variation on recognition by monoclonal antibodies neutralize simian immunodeficiency virus infectivity. Journal of Virology 68(9): 5395-5402

The determinants of immune recognition by five monoclonal antibodies (KK5, KK9, KK17, Senv7.1, and Senv101.1) that neutralize simian immunodeficiency virus infectivity were analyzed. These five neutralizing monoclonal antibodies were generated to native SIVmac251 envelope glycoprotein expressed by a vaccinia virus recombinant vector. All five recognize conformational or discontinuous epitopes and require native antigen for optimal recognition. These monoclonal antibodies also recognize SIVmac239 gp120, but they do not recognize gp120 of two natural variants of SIVmac239, 1-12 and 8-22, which evolved during the course of persistent infection in vivo (D.P.W. Burns and R.C. Desrosiers, J. Virol. 65:1843-1854, 1991). Recombinant viruses which were constructed by exchanging variable regions between SIVmac239 and variant 1-12 were used to define domains important for recognition. Radioimmunoprecipitation analysis demonstrated that sequence changes in variable regions 4 and 5 (V4/V5) were primarily responsible for the loss of recognition of the 1-12 variant. Site-specific mutants were used to define precise changes that eliminate recognition by these neutralizing antibodies. Changing N-409 to D, deletion of KPKE, and deletion of KEQH in V4 each resulted in loss of recognition by all five monoclonal antibodies. SIVs with these natural sequence changes are still replication competent and viable. Changing A-417 to T or A/N-417/418 to TK in V4 or Q-477 to K in V5 did not alter recognition detectably. These results define specific, naturally occurring sequence changes in V4 of SIVmac that result in loss of recognition by one class of SIVmac neutralizing antibodies.

US$29.90

PMID: 7520089


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Antabak, A.; Skegro, M.; Batinica, S.; Zupancić, B.; Popović, L., 2009: Laparoscopic cholecystectomy in a child with sickle cell disease. Laparoscopic cholecystectomy has become the procedure of choice for the removal of the diseased gallbladder in children. Its use in small children and infants is rare. At the Department of Pediatric Surgery of the Medical faculty in Zagreb five ch...

Miguez, M.W.; Mcfadden, A.; Kornstein, M.J.; Harris, A.; Ben Ezra, J.; Neifeld, J., 1994: Primary gastrointestinal non-Hodgkins lymphomas A clinicopathologic review of 107 cases. Modern Pathology 7(1): 64A

Imani-Nasab, M.Hasan.; Seyedin, H.; Majdzadeh, R.; Yazdizadeh, B.; Salehi, M., 2014: Development of evidence-based health policy documents in developing countries: a case of Iran. Evidence-based policy documents that are well developed by senior civil servants and are timely available can reduce the barriers to evidence utilization by health policy makers. This study examined the barriers and facilitators in developing evid...

Jeffreys, J.G., 1877: Address to the Biological Section of the British Association at Plymouth. Unknown

Ongley, P.A., 1953: The alkaloids of Mitragyna. Annales Pharmaceutiques Francaises 11(9-10): 594-602

Sánchez-Quijano, A.; Pineda, J.A.; Lissen, E., 1988: Post-transfusion hepatitis. A pending issue. Medicina Clinica 91(3): 117-118

Laiken, S.L.; Johnson, A.D.; Bhargava, V.; Rigo, P., 1979: Instantaneous transmitral blood flow and anterior mitral leaflet motion in man. Transmitral blood flow was measured in man by numerical differentiation of left ventricular volume as a function of time in 11 patients undergoing cardiac catheterization. Using this technique, transmitral blood flow may be studied in a variety of...

Xiang, X.; Zhang, G.; Wang, X.; Tang, T.; Shi, Y., 2015: A new perspective on the process of intrinsic point defects in α-Al₂O₃. First-principles plane-wave pseudopotential calculations have been performed to study the charge states and energetics of intrinsic point defects as vacancies, interstitials and antisite atoms in α-Al2O3, and thus a new perspective on the process...

Itani, O.A.; Flibotte, S.; Dumas, K.J.; Guo, C.; Blumenthal, T.; Hu, P.J., 2016: N-Ethyl-N-Nitrosourea (ENU) Mutagenesis Reveals an Intronic Residue Critical for Caenorhabditis elegans 3' Splice Site Function in Vivo. Metazoan introns contain a polypyrimidine tract immediately upstream of the AG dinucleotide that defines the 3' splice site. In the nematode Caenorhabditis elegans, 3' splice sites are characterized by a highly conserved UUUUCAG/R octame...

Taggart, D.J.; Camerlengo, T.L.; Harrison, J.K.; Sherrer, S.M.; Kshetry, A.K.; Taylor, J-Stephen.; Huang, K.; Suo, Z., 2013: A high-throughput and quantitative method to assess the mutagenic potential of translesion DNA synthesis. Cellular genomes are constantly damaged by endogenous and exogenous agents that covalently and structurally modify DNA to produce DNA lesions. Although most lesions are mended by various DNA repair pathways in vivo, a significant number of damage...