geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of neocarzinostatin on chromatin in HeLa S3 nuclei


, : Effects of neocarzinostatin on chromatin in HeLa S3 nuclei. Molecular Pharmacology 23(2): 493-499

Neocarzinostatin solubilizes chromatin from HeLa S3 nuclei by introducing strand scissions in linker regions. Multimeric nucleosome patterns are seen on both native and denaturing gel analysis. The mechanism of drug action differs from the type of chromatin digestion seen with micrococcal enzyme in that DNA damage occurs through single-strand breaks and less acid-soluble material is produced. In addition, drug-induced release of soluble chromatin from the nuclei is not very dependent upon the addition of EDTA. The monomer repeat size is larger than that found for micrococcal enzyme and contains linker regions that are partially single-stranded. Core histone proteins as well as histone H1 do not appear to be altered by drug action, although there is clear evidence that DNA damage can occur in nucleosome cores. The chromophore portion of the drug degrades chromatin as effectively as the holoantibiotic.

US$29.90

PMID: 6220204


Other references

Lasser, E., 1958: Experiences in the combination breeding of winter and spring barleys. The improvement of winter hardiness, standing capacity, disease resistance and other important characters is discussed in relation to the author's own hybridization experiments in Austria.

Mühe, E.; Gentsch, H.H.; Hager, T.H., 1977: Treatment of ulcerative colitis and Crohn's disease. Medizinische Monatsschrift 31(5): 209-212

Nokleberg W.J.; Scotese C.R.; Bundtzen T.K.; Parfenov L.M.; Monger J.W.H.; Dawson K.M.; Khanchuk A.I.; Goryachev N.A.; Shpikerman V.I.; Norton I.O., 2002: Dynamic computer model for the metallogenesis and tectonics of the Circum-North Pacific. The metallogenic and tectonic development of the Circum-North Pacific (Russian Far East, Alaska, Canadian Cordillera, and adjacent offshore areas) is illustrated in a dynamic computer model that portrays the formation of a series of metallogenic b...

Mathieson, P.W., 1996: Immune modulation strategies in T cell mediated autoimmune diseases-relevance to IDDM. Journal of Pediatric Endocrinology & Metabolism 9 Suppl 1: 133-138

VOLOKHOV.A.A.; ORBELI.L.Argarovich., 1957: Leon Abgarovich Orbeli; 75th anniversary of his birth. Biulleten' Eksperimental'noi Biologii i Meditsiny 43(6): 109-113

Blair C.E., 1975: Effect of vaso constrictive stimuli on pulmonary shunt flow in dogs with granulomatous pulmonary disease. Federation Proceedings: 417

Vigneaux M., 1969: Burdigalian stratotype. Giornale di Geologia (Bologna) 37(2): (1971)

McDowell Ronald R., 1986: Middle Ordovician Kanosh Shale; an overlooked Great Basin source rock. AAPG Bulletin: , Pages 1048. 1986.

Coon, J.J.; Steele, H.A.; Laipis, P.J.; Harrison, W.W., 2003: Direct atmospheric pressure coupling of polyacrylamide gel electrophoresis to mass spectrometry for rapid protein sequence analysis. Using laser desorption-atmospheric pressure chemical ionization we describe a novel approach for coupling mass spectrometry to polyacrylamide gel electrophoresis. In contrast to other approaches, the method allows for the direct sampling of a poly...

Tesdal, I.K.; Jaschke, W.; Bühler, M.; Adamus, R.; Filser, T.; Holm, E.; Georgi, M., 1997: Transjugular intrahepatic portosystemic shunting (TIPS) with balloon-expandable and self-expanding stents: technical and clinical aspects after 3 1/2 years' experience. To evaluate prospectively our experience with transjugular intrahepatic portosystemic shunt (TIPS) using four different metallic stents. Between November 1991 and April 1995, 57 patients (41 men and 16 women; age 35-72 years, mean 54 years) underw...