+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of nephrectomy and KC1 on transmembrane potentials, intracellular electrolytes, and cell pH of rat muscle and liver in vivo

, : Effects of nephrectomy and KC1 on transmembrane potentials, intracellular electrolytes, and cell pH of rat muscle and liver in vivo. Journal of Physiology 212(1): 117-128

1. The ability of 24 hr nephrectomy and KCl to raise plasma K(+) concentration was used to correlate transmembrane resting potential (RP), ionic gradients, and cell pH (DMO method) in rat muscle and liver in vitro.2. Effects of 24 hr nephrectomy on electrolytes included a rise in plasma K(+) and fall in Na(+), with a rise in intracellular K(+) and fall in intracellular Na(+) in both liver and muscle. Intracellular Cl(-) concentration rose in muscle and decreased in liver.3. Measured muscle RP was decreased from -91 to -77 mV by 24 hr nephrectomy, with the RP predictable from the Goldman equation for Na(+) and K(+) with P(Na)/P(K) = 0.01 and Cl(-) behaving as if passively distributed.4. Measured hepatic RP was increased from -43 to -48 mV by 24 hr nephrectomy, with a change in ionic permeability or activation of an electrogenic pump necessary to explain the results.5. Plasma acid-base changes consisted of metabolic acidosis with partial respiratory compensation. Cell pH rose slightly in both liver and muscle; the H(+) gradient remained constant in muscle but increased slightly in liver.6. KCl was injected into intact rats while the RPs were continuously measured in muscle or liver. Muscle RP was found to decrease and hepatic RP to increase along a similar time course.7. Infusion of KCl into the portal vein led to an increase in the hepatic RP for values of hepatic venous K(+) of 15-25 mM, whereas infusion sufficient to increase the hepatic venous K(+) concentration to 30-40 mM produced no change or a slight decrease in hepatic RP.8. The rat muscle RP can be adequately described by the Goldman equation for Na(+) and K(+), whereas the hepatic RP may well have both diffusion and electrogenic components which respond differently to an increase in plasma K(+) concentration.


PMID: 5545176

Other references

Dubost G., 1975: Behavior of the african chevrotain hyemoschus aquaticus artiodactyla ruminantia ecologic and phylogenetic significance. Zeitschrift fuer Tierpsychologie 37(4): 403-448

Konarev A.V., 1985: Methods for analyzing the composition of cereal alpha amylase and proteinase inhibitors. A complex of methods is proposed for studying various types of enzyme inhibitors from wheat and other cereals. Inhibitors of human, insect and cereal .alpha.-amylases were revealed among wheat grain proteins fractionated by gel electrophoresis, is...

Brink, H.M.; Beex, L.V., 1995: Punctal and canalicular stenosis associated with systemic fluorouracil therapy. Report of five cases and review of the literature. Ocular side effects of systemic fluorouracil include excessive lacrimation, due to punctal and canalicular stenosis and fibrosis. Obstruction of the tear ducts after systemic therapy with fluorouracil is more frequent than is assumed. Five patient...

Anonymous, 2013: Issue information. Physiological Reports 1(2): E000i0-E000i0

Corelli R.L.; Haas J.; Guglielmo B.J., 1991: Prior treatment with third generation cephalosporins tgc is associated with subsequent isolation of tgc resistant gram negative rods. Program & Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 31: 266

Grant, M.B.; Guay, C.; Lottenberg, R., 1988: Desmopressin stimulates parallel norepinephrine and tissue plasminogen activator release in normal subjects and patients with diabetes mellitus. Desmopressin acetate administration markedly stimulates release of tissue plasminogen activator (t-PA) from vascular endothelial cells. The mechanism for this effect is unknown. Because infusion of epinephrine has been shown to increase t-PA level...

Rodrigues Rene; Takaki Tikae, 1987: Isotope stratigraphy of Cenozoic carbonate sequences of the Brazilian northern coast. Boletim de Geociencias da PETROBRAS 1(2): 177-182

Cagini, P.; Diamantini, G., 1970: Congenital diffuse malignant keratoma. Minerva Pediatrica 22(12): 572-576

Ezeala, D.O.; Hart, J.W.; Sabnis, D.D., 1974: Fractionation of monovalent ion-stimulated nucleoside triphosphatase activity in extracts of petiolar tissues. Extracts of isolated phloem and xylem tissues of Heracleum mantegazzianum contained 2 and 1 enzyme, respectively, capable of hydrolysing ATP. Enzyme I, common to both extracts, showed preferential hydrolysis of nucleoside triphosphates and was sti...

Techo, S.; Miyashita, M.; Shibata, C.; Tanaka, N.; Wisetkhan, P.; Visessanguan, W.; Tanasupawat, S., 2016: Lactobacillus ixorae sp. nov., isolated from a flower (West-Indian Jasmine). NlmCategory="UNASSIGNED">A Gram-stain positive, lactic acid bacterium strain Ru20-1T was isolated from a flower (West-Indian Jasmine) collected from Kalasin province, Thailand. Polyphasic approaches were used to determine the taxonomi...