+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of neuraminidase treatment on CFUs properties and CFUs numbers

, : Effects of neuraminidase treatment on CFUs properties and CFUs numbers. Experimental Hematology 10(4): 393-403

Incubation of bone marrow cells with Vibrio cholerae neuraminidase decreases the number of CFUs to a minimum of 25% of control numbers. It is shown that this decrease in CFUs numbers is not due to cytotoxic effects of neuraminidase but to a decrease in the spleen seeding efficiency of colony forming cells. Neuraminidase acts in two ways. It removes negatively charged neuraminic acids from membrane glycoproteins and glycolipids and it can adsorb to the cell surface. It is investigated which of the two enzyme effects is responsible for the decrease in CFUs numbers. Inhibition of the enzyme reaction by EDTA prevents to a great extent the decrease in CFUs numbers, while tests with mouse spleen cells and human erythrocytes show that EDTA has no or a positive effect on the adsorption of neuraminidase to the cell surface. This indicates that the neuraminidase induced decrease in CFUs numbers is caused by the enzymatic removal of neuraminic acids rather than by the adsorption of the enzyme to the cell surface.


PMID: 6807703

Other references

Furber, A.; Merheb, M.; Boulet, S.; Prunier, F.; Pézard, P.; L'Hoste, P.; Tadei, A.; Geslin, P., 1999: Variations of coronary blood flow measured by intracoronary doppler in the acute phase of myocardial infarction before and after revascularization by angioplasty. The authors studied the changes in coronary blood flow before, during and after reperfusion by angioplasty of the disease coronary vessel responsible for myocardial infarction using intracoronary Doppler. Forty patients aged 60.2 +/- 15.4 years, a...

Yawetz, A.; Agosin, M.; Perry, A.S., 1978: Components of the electron transport system in the microsomal mixed function oxidase system in wild birds. Notable differences were found among 6 spp. [Passer domesticus, Turdus merula, Pycnonotus capensis, Sturnus vulgaris, Alectoris graeca, Tyto alba] of wild-caught birds in the levels of cytochrome P-450, cytochrome b5, NADPH-cytochrome c reductase...

Silva P.; Eveloff J.; Koenig B.; Kinne R., 1980: Cyclic amp controls respiration in cells of medullary thick ascending limb of henles loop. Clinical Research 28(2): 461A

Pedroza, A.; Mosqueda, R.A.onso-Vante, N.R.driguez-Vazquez, R., 2007: Sequential treatment via Trametes versicolor and UV. Chemosphere 67(4): 793-801

Ermolenko, A.E.; Perepada, E.A., 2006: Origin of segmentation in the human structure. Crystallographic analysis of biological and non-biological minerals does not reveal any significant differences between the two, which is indicative of common crystallization processes. It can be supposed that the human organism is a biocrystalloi...

Jin, Z.; Wen, Y.; Xiong, L.; Yang, T.; Zhao, P.; Tan, L.; Wang, T.; Qian, Z.; Su, B-Lian.; He, Q., 2017: Intratumoral H 2 O 2 -triggered release of CO from a metal carbonyl-based nanomedicine for efficient CO therapy. A new H<sub>2</sub>O<sub>2</sub>-responsive nanomedicine for CO therapy is constructed by effectively encapsulating the hydrophobic manganese carbonyl prodrug into an advanced hollow mesoporous silica nanoparticle carrier t...

Li, N.; Zhang, Q-Yue.; Zou, J-Ling.; Li, Z-Wu.; Tian, T-Tian.; Dong, B.; Liu, X-Juan.; Ge, S.; Zhu, Y.; Gao, J.; Shen, L., 2016: miR-215 promotes malignant progression of gastric cancer by targeting RUNX1. miR-215 was reported to be downregulated and functioned as a tumor suppressor in several cancers. In contrast, miR-215 was preferentially upregulated in gastric cancer (GC) according to our data. Thus, we studied the potential biological function...

Savchenko, E.N.; Violovich, N.A., 1967: Review of the crane-flies (Diptera, Tipulidae) of the Tuva. Annales Zoologici Warszawa, 25: 317-365

Hawkins, R.H.; Cundy, T.W., 1987: Steady-state analysis of infiltration and overland flow for spatially-varied hillslopes. An envelope of steady-state surface runoff response for a hillslope is established in terms of the probability distribution and spatial arrangement of individual point infiltration capacities and the rainfall intensity. Minimum overland flow is sh...

High, W.A.; Larson, M.J.; Hoang, M.P., 2004: Idiopathic bilateral auricular ossificans: a case report and review of the literature. Petrification of the auricle results in a rigid and immalleable ear. The etiology of such a finding is usually ectopic calcification. The condition has been associated with injurious processes, such as cold injury, and with various endocrinopathie...