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Electron microscopic mapping of wheat germ RNA polymerase II binding sites on cloned CaMV DNA

, : Electron microscopic mapping of wheat germ RNA polymerase II binding sites on cloned CaMV DNA. Nucleic Acids Research 9(16): 3927-3939

The binding sites of wheat germ RNA polymerase II were mapped on the cloned CaMV genome by observation of enzyme-linear DNA complexes by electron microscopy. Twelve sites are observed. Three of them are relatively stable in the presence of heparin and are found at positions 8-9, 21-23, and 41-44 map units on the physical map of the genome. These positions correspond to AT-rich regions of the viral genome which contain potential promoter sites. These results are discussed with reference to current information on the structure and expression of the CaMV genome.


PMID: 7301575

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