+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Glycoprotein synthesis in explants of developing rabbit mammary gland in culture

, : Glycoprotein synthesis in explants of developing rabbit mammary gland in culture. Biochemical Journal 198(3): 683-690

1. Explants of mammary glands of pregnant rabbits cultured in the absence of insulin, prolactin and cortisol incorporated [2-3H]mannose into lipid-linked mono- and oligo-saccharide and protein. 2. Inclusion of the hormones in the culture medium stimulated the incorporation of [2-3H]mannose into lipid-linked monosaccharide 4-fold, into lipid-linked oligosaccharide 4-fold and into protein 13-fold after 24 h in culture. 3. Addition of tunicamycin to the incubation medium completely inhibited the incorporation of [2-3H]mannose into lipid-linked oligosaccharide and protein after an initial lag period of about 2h. Incorporation of this radiolabel into lipid-linked monosaccharide was increased 4-fold under these conditions. 4. Incorporation of [4,5-3H]leucine into protein was unaffected by the presence of tunicamycin. 5. Analysis of mannose-labelled protein by polyacrylamide-gel electrophoresis indicated that a major radiolabelled protein of apparent mol.wt. 65,000-70,000 was synthesized and approx. 70% of this protein appeared in the soluble fraction. 6. Glycosylation of the protein but not synthesis of its peptide backbone was sensitive to tunicamycin. 7. Possible origins of this glycoprotein synthetized when the tissue is stimulated to differentiate in culture are discussed.


PMID: 7326034

Other references

Mao, Y.; Horn, P.R.; Mardirossian, N.; Head-Gordon, T.; Skylaris, C-Kriton.; Head-Gordon, M., 2016: Approaching the basis set limit for DFT calculations using an environment-adapted minimal basis with perturbation theory: Formulation, proof of concept, and a pilot implementation. Recently developed density functionals have good accuracy for both thermochemistry (TC) and non-covalent interactions (NC) if very large atomic orbital basis sets are used. To approach the basis set limit with potentially lower computational cost,...

Hrivnák, Ján.; Tölgyessy, P.; Královicová, E., 2009: On-column injection system for use of large diameter inside needle capillary adsorption trap devices. Direct coupling of the needle trap to the capillary column in the split/splitless gas chromatographic (GC) inlet with simple modification facilitates full exploitation of the column efficiency, even when using the large volume trap filled with 35...

Gauvry, L.; Ennion, S.; Hansen, E.; Butterworth, P.; Goldspink, G., 1996: The characterisation of the 5' regulatory region of a temperature-induced myosin-heavy-chain gene associated with myotomal muscle growth in the carp. The 5' region of a myosin heavy chain gene was isolated from carp (Cyprinus carpio) and characterized. It was previously shown that gene expression is induced by an increase in environmental temperature and is restricted to the small-diameter...

Rovellini A.; Sommariva D.; Branchi A.; Maraffi F.; Montalto C.; Gandini R.; Fasoli A., 1992: Effects of slow release bezafibrate on the lipid pattern and on blood glucose of type 2 diabetic patients with hyperlipidaemia. Ninety-eight type 2 diabetic patients with hyperlipidaemia in stable metabolic control with diet alone (41) or diet plus hypoglycaemic agents (57) were divided into two groups: group 1 was put on treatment with slow release bezafibrate 400 mg a da...

Havenaar, D., 2004: Preliminary figures point to a large decrease in apple area. Preliminary Dutch land use data for 2003 showed a decrease in the area grown with apples (-7.9%) and small fruits (-10.8%), a small increase in the are for pears (+0.9%) and fruits for industrial processing (+4.8%), and almost a doubling of fruits...

Antunes, J.; Prates, S.; Leiria-Pinto, P., 2015: Fixed drug eruption due to etoricoxibe - a case report. Allergologia et Immunopathologia 42(6): 623-624

Beyens, L.; Chardez, D. de Baere, D., 1990: Ecology of terrestrial testate amoebae assemblages from coastal lowlands on Devon Island (NWT, Canadian Arctic). Fifty-seven taxa belonging to 17 genera of testate rhizopods were recorded in samples of mosses, soils and lichens, collected from the Truelove and Sparbo Hardy Lowlands on the high arctic Devon Island (NWT, Canada). Different groups of habitats a...

Vassout A.; Bischoff S., 1987: Hypothermic effects of neurotensin nt and analogs comparison with nt receptor interaction. Experientia (Basel) 43(6): 651

Beauvallet, C.R.nou, J., 1992: Applications of NMR spectroscopy in meat research. Trends in food science and technology 3(8-9): 241-246

Erb, C.; Messerli, J.; Flammer, J., 1998: Latanoprost: A new prostaglandin F2alpha analogue in glaucoma therapy. A survey. An ideal glaucoma drug reduces intraocular pressure profoundly and long-lastingly without known major side effects. Latanoprost, a new prostaglandin F2alpha-analogue, indeed has these properties. However, we need long-term experience to exclude ma...