+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

High glucose and TGF beta 1 stimulate fibronectin gene expression through a cAMP response element

, : High glucose and TGF beta 1 stimulate fibronectin gene expression through a cAMP response element. Kidney International 46(4): 1019-1024

Previously, we reported that mesangial cells increased fibronectin, laminin and type IV collagen synthesis when cultured in the presence of high glucose (30 mM). Although mRNA levels for all three extracellular matrix (ECM) proteins were also increased in high glucose conditions, the mechanism for this increase was not known. In order to determine whether increased transcription was involved in the observed increase in fibronectin mRNA levels mesangial cells were transfected with a construct containing the 5'-flanking region of the fibronectin (FN) gene [position +69 to -510 base pairs (bp)] fused to the coding region of the chloramphenicol acetyltransferase (CAT) gene [FN-CAT (-510)]. Cells were transiently and stably transfected with this construct. Under serum-free conditions, high glucose increased CAT activity only in the presence of TGF beta 1 (referred to as TGF beta). The experiments were performed without serum because FN-CAT (-510) contains a serum responsive element. The increase in CAT was approximately twofold in transiently transfected cells and threefold in stably transfected cells. TGF beta alone increased CAT activity approximately 30%. Stimulation of fibronectin gene expression appeared to occur at the level of a cAMP response element (CRE) located -170 bp of the FN gene because cells transfected with a construct containing an oligonucleotide encoding for this CRE fused to a minimal fibronectin promoter (-56 bp) and a CAT reporter gene [CRE (-170) FN-CAT] displayed similar increments of CAT activity after treatment with high glucose and TGF beta. Gel shift mobility assays with a CRE oligonucleotide revealed multiple complexes with mesangial cell nuclear proteins.


PMID: 7861696

Other references

Fassett, Norman C., 1953: North American Ceratophyllum. Several different series of variation are imposed on the well known basic pattern of leaves and fruits of Ceratophyllum. The purpose of this study was to determine which patterns represent differences between species and which are variations withi...

Kohen Raz, R.; Roth, V., 2000: Posturographic characteristics of whiplash patients. Claussen, Claus-Frenz [Author], Haid, Claus-Toni [Author], Hofferberth, Bernhard [Author] International Congress Series; Equilibrium research, clinical equilibriometry and modern treatment 647-660

Zaoral, J.; Kvapilik, J.; Krnavek, B., 1988: Improvement of fertility of cows inseminated in synchronised oestrus. Data were obtained over a 4-yr period on a herd comprising 100 Holstein-Friesian X Czech Pied cows. The cows were inseminated at predetermined intervals following oestrus synchronization with cloprostenol. The CR to 1st insemination in the 4 test...

Chaudhry, K.M.; Sher Muhammad; Ijaz Ashraf, 2006: Alternative extension approaches to technology utilization for sustainable agriculture in the Punjab, Pakistan. Technology utilization by farmers is an important factor, which can boost agricultural production. For this purpose, extension agencies need to put more efforts in creating awareness and facilitating the farmers for the utilization of agricultural...

Stoyanov, I.; Ljubomirov, T., 1997: A preliminary faunistic list and zoogeographical analysis of the gold and digger wasps Hymenoptera Chrysididae, Sphecidae from the Rila Mountain. Acta Entomologica Bulgarica, 33-4: 24-29

Beger, Hans, G., 2003: Preventive surgery: The impact of molecular biology in surgery. Langenbeck's Archives of Surgery 388(1): 1-2

Anonymous, 1994: Dutch Society of Gastroenterology and Dutch Society of Hepatology meeting. Veldhoven, 7-8 October 1993. Abstracts. Netherlands Journal of Medicine 44(2): A11-A44

Jones P.M.; Stutchfield J.; Howell S.L., 1985: Effects of calcium and a phorbol ester on insulin secretion from islets of langerhans permeabilized by high voltage discharge. Febs Letters 191(1): 102-106

Segers, P.; Leather, H.Alex.; Verdonck, P.; Sun, Y-Yuan.; Wouters, P.F., 2002: Preload-adjusted maximal power of right ventricle: contribution of end-systolic P-V relation intercept. To assess whether preload-adjusted maximal power (PAMP), which is calculated as Wmax/Vedb (where Wmax is maximal power and Ved is end-diastolic volume with b = 2) is an index of right ventricular (RV) contractility, we measured RV pressure (P) and...

Silvain, C.; Besson, I.; Beauchant, M., 1994: Somatostatin, octreotide and portal hypertension. Gastroenterologie Clinique et Biologique 18(8-9): 761-766