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In vitro-isolated human cytotoxic T-lymphocyte clones detect variations in serologically defined HLA antigens

, : In vitro-isolated human cytotoxic T-lymphocyte clones detect variations in serologically defined HLA antigens. Immunogenetics 16(6): 503-512

T cells of two donors, JR (HLA-A23,29;B7,7;C-;DRw5) and HG (HLA-A2,23;B40,w44;Cw4), were stimulated with cells from an HLA homozygous lymphoblastoid cell line JY (HLA-A2,2;B7,7,C-,DRw4,6) and cloned by limiting dilution after the third stimulation. Two cytotoxic T-cell (CTL) clones, JR-2-16 (from donor JR) and HG-31 (from donor HG), were used for detailed studies. The results of a panel study using lymphocytes from HLA-typed individuals and a study with two HLA recombinant families indicate that the antigens recognized by the CTL clones JR-2-16 and HG-31 were highly associated with HLA-A2 and HLA-B7, respectively. Blocking studies with a monoclonal antibody recognizing a framework determinant on HLA-A, -B and -C antigens and a monoclonal antibody reacting with HLA-A2 support the notion that JR-2-16 and HG-31 interact with the HLA-A2 and the HLA-B7 antigens per se. However, these clones did not recognize the HLA-A2 and HLA-B7 of all donors typed for these antigens, suggesting that the HLA-A2 and HLA-B7 antigens of these particular donors are variants of the serologically defined HLA antigens. These results indicate that in vitro-derived human CTL clones detect variations in the serologically defined allospecificities and can be used as reagents to elucidate the polymorphism of HLA antigens further.


PMID: 6190736

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Arretz, P.; Santis, L. de; Guerrero, M.A.; Lamborot, L., 1989: Presence of Aprostocetus daira (Walker) (Hymenoptera: Eulophidae) in Chile. Larvae of Aprostocetus daira were found parasitizing the apid lucerne pollinator Megachile rotundata for the first time in Chile in 1984-85. Details on the taxonomy and aspects of the biology of the eulophid are given. The parasitoid stayed in dia...

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Jayathangaraj, M.G.; John, M.C.; Gopalakrishnan, A.V., 1998: Acaricidal trial on rat snakes. A trial was conducted to evaluate the efficacy of carbaryl dust and amitraz solution as acaricides in Elaphe obsoleta. 2 E. obsoleta infested with Aponomma sp. ticks were either dusted with carbaryl powder or amitraz was applied. The snakes were s...

Vial, H.J.; Angelin, M.L.; Elabbadi, N.; Calas, M.; Cordinas, G.; Giral, L., 1993: Basic biochemical investigations as rationale for the design of original antimalarial drugs An example of phospholipid metabolism. Memorias do Instituto Oswaldo Cruz Rio de Janeiro 87(SUPPL 3): 251-261, 1992

Segeren, C.M.; Polderman, K.H.; Lips, P., 1997: Agenesis of the corpus callosum associated with paroxysmal hypothermia: Shapiro's syndrome. Spontaneous recurrent hypothermia and hyperhidrosis associated with agenesis of the corpus callosum was first described by Shapiro and Plum in 1967. Since then, several cases with similar symptoms (now known as Shapiro's syndrome or spontaneo...

Cheshik, S.G.; Vartanian, R.V.; Ivanova, L.A.; Kisteneva, L.B.; Galegov, G.A., 1992: The use of a virazole aerosol in young children with a respiratory syncytial virus infection. The therapeutic effect of aerosol virasol was studied in young infants suffering from RS virus infection with concomitant broncho-obstructive syndrome. The study involved 60 children between 1 month and 4 years of age of whom 30 were treated with...

Kaplan, J.E.; Masur, H.; Holmes, K.K.; McNeil, M.M.; Schonberger, L.B.; Navin, T.R.; Hanson, D.L.; Gross, P.A.; Jaffe, H.W., 1995: USPHS/IDSA guidelines for the prevention of opportunistic infections in persons infected with human immunodeficiency virus: introduction. USPHS/IDSA Prevention of Opportunistic Infections Working Group. Clinical Infectious Diseases 21 Suppl 1: S1-11

Simon, M.; L'Heveder, G.; Genestet, S.; Misery, L., 2010: Efficacy of capsaicin in the treatment of acne in one case: new pathophysiological perspectives. Journal of the European Academy of Dermatology and Venereology 24(7): 857-857

Horstmann, K., 1969: Typenrevision der europaischen Arten derGattung Diadegma Foerster (syn. Angitia Holmgren.) (Hymenoptera: Ichneumonidae). Beitr. Ent.: 19: 413-472

Tsao, M.U.; Madley, T.I., 1975: Regulation of glycolysis in Neurospora crassa. Kinetic properties of pyruvate kinase. Pyruvate kinase (ATP:pyruvate phosphotransferase, EC, extracted from the mycelium of Neurospora crassa has been purified 560-fold by precipitation with ammonium sulphate, chromatography with DEAE-Sephadex, and gel filtration with Sephade...