geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Induction of macrophage-mediated cytolysis of neoplastic cells by mycoplasmas


, : Induction of macrophage-mediated cytolysis of neoplastic cells by mycoplasmas. Cellular Immunology 77(2): 290-297

Unexpected cytolysis was encountered when nonactivated murine peritoneal macrophages were cultured with [3H]TdR-prelabeled syngeneic or allogeneic tumor cells at a 10:1 ratio. The level of specific cytolysis reached 70% within 48 hr of cocultivation. Similar killing was observed whether the macrophages were derived from untreated, thioglycollate-treated, or germ-free mice. Cytolytic activity was also demonstrated when bone marrow-derived or peritoneal macrophages from 9- and 5-day in vitro cultures, respectively, were employed rather than freshly harvested peritoneal macrophages. Thus, the macrophage-mediated killing was neither the result of in vivo preactivation nor a consequence of the presence of lymphocytes in the assay. Moreover, macrophages derived from different strains caused similar effects. Our study revealed that the neoplastic target cell cultures susceptible to cytolysis by nonactivated macrophages were contaminated with mycoplasma. A mycoplasma was isolated from the supernatant of a culture of the A9HT fibrosarcoma line, identified as Mycoplasma orale, and cultivated. Addition of viable mycoplasma from that isolate to mixed cultures of thioglycollate-elicited macrophages and [3H]TdR-prelabeled mycoplasma-free target cells resulted in specific cytolysis of transformed A9 cells, but not of normal mouse fibroblasts. The level of macrophage-dependent cytolysis correlated with the number of viable mycoplasma cells added and was higher than that attained by activation with LPS at optimal concentration. Similar specific cytolysis was observed with heat-killed mycoplasmas. Our results demonstrate that mycoplasmas may cause selective macrophage-mediated cytolysis of neoplastic but not of normal target cells, perhaps via activation of the macrophages. It is suggested that undetected infection of experimental systems by mycoplasmas may account for some reports on lysis of neoplastic cells by nonactivated macrophages.

US$29.90

PMID: 6850843


Other references

Anonymous, 1973: Research into domestication, and the history of domestic animals. International symposium held in Budapest in 1971. All but four of the papers delivered at the symposium are reproduced in the present volume. They have been grouped, according to subject, under the following headings: I. Biological and historical problems of domestication; II. Investigations into...

Summers, D.; Frese, K.J., 1988: Mechanistic aspects of the electrochemical reduction of carbon monoxide and methanol to methane at ruthenium and copper electrodes. ACS Symposium series American Chemical Society: 78) 518-527

Stone, J.M., 1971: J.C.A.H. standards emphasize better management, physician participation. Modern Hospital 116(2): 108-10 Passim

Zardalishvili, 1957: Side-dressing sugar beets with boron. Action of boracic fertilizers on sugar beets and on their sugar content was studied. Experiments were conducted by the Academy of Sciences of the Georgian SSR in alluvial-meadow carbonaceous soil under conditions of irrigation. Boron in form of bo...

Rogelj, I., 1992: Growth and proteolytic activity of Pseudomonas fluorescens isolated from milk. The relationship between growth kinetics of Pseudomonas fluorescens 33, and its proteolytic activity in raw and sterilized milk stored at 7 degrees C was studied. Kinetics were determined by experimental growth curves. The proteolytic activity was...

Cook, J., 1953: Further notes on the marketing of smallholding latex. Further experience on the handling of smallholders' latex has shown that a system involving both latex collection and processing is likely to have a more stable future than the operation of collecting centres disposing of latex as latex. At p...

Serra, G.E., 1963: Variations In The Hematocrit And Hemoglobin In The Puerperium. Quaderni di Clinica Ostetrica E Ginecologica 18: 1049-1055

Opwora, A.; Kabare, M.; Molyneux, S.; Goodman, C., 2011: Direct facility funding as a response to user fee reduction: implementation and perceived impact among Kenyan health centres and dispensaries. There is increasing pressure for reduction of user fees, but this can have adverse effects by decreasing facility-level funds. To address this, direct facility funding (DFF) was piloted in Coast Province, Kenya, with health facility committees (HF...

Saxod R., 1970: Study under the electron microscope of the histogenesis of the cutaneous sensory cells of herbst and gandry ducks beak. Annee Biologique 9(7-10): 589-590

Ahmad, S.; Khan, Z.U.; Johny, M.; Ashour, N.M.; Al-Tourah, W.H.; Joseph, L.; Chandy, R., 2013: Isolation of Lodderomyces elongisporus from the Catheter Tip of a Fungemia Patient in the Middle East. Lodderomyces elongisporus is phenotypically closely related to Candida parapsilosis and has recently been identified as an infrequent cause of bloodstream infections in patients from Asia and Mexico. We report here the isolation of Lodderomyces el...