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Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC


, : Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC. International Journal of Peptide and Protein Research 22(1): 78-82

The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion-pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine-containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion-pairing of trifluoroacetate with the histidine residues of the tryptic peptides.

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PMID: 6885251


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