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Localization of Met-enkephalin-like immunoreactivity within pain-related nuclei of cervical spinal cord, brainstem and midbrain in the cat


, : Localization of Met-enkephalin-like immunoreactivity within pain-related nuclei of cervical spinal cord, brainstem and midbrain in the cat. Brain Research Bulletin 11(5): 587-604

Met-enkephalin immunoreactivity was investigated with an indirect immunoperoxidase technique in the cervical spinal cord, brainstem and midbrain of the cat, paying special attention to pain-related nuclei. Different technical conditions were used to reveal preferentially met-enkephalin-containing fibres and terminals or perikarya. Immunoreactive fibres and terminals were revealed optimally in sections from control animals incubated with detergent (Triton X-100). Immunoreactive perikarya were revealed in colchicine treated animals. Comparison between different routes of administration showed that local injections of colchicine are needed to reveal optimally immunoreactive perikarya in nuclei located far from the ventricles. Met-enkephalin-containing fibres and terminals are widely distributed in the posterior brain and spinal cord. The densest network of immunoreactive fibers are observed in the superficial layers of the cervical spinal cord and the caudal trigeminal nucleus, in the nucleus of the solitary tract, the nucleus of the facial nerve, the nucleus of the prepositus hypoglossi, the nucleus raphe pallidus, the medial vestibular nucleus, the interpedoncular nucleus and the substantia nigra. A moderate staining of fibres is observed in various nuclei including the ventral horn of the spinal cord and caudal trigeminal nucleus, the brainstem and midbrain reticular formation, the inferior olivary complex, the nucleus of the descending trigeminal tract and the periaqueductal grey. Met-enkephalin-containing perikarya are present in all the nuclei cited before, except in the inferior olivary complex. The densest aggregation of enkephalin-like perikarya is observed in the nucleus raphe magnus, nucleus raphe obscurus, nucleus raphe pallidus, nucleus reticularis gigantocellularis pars alpha and nucleus reticularis lateralis. The general distribution of enkephalin-containing structures in the cervical spinal cord, brainstem and midbrain of the cat appears very similar to that of the rat except in the substantia nigra where met-enkephalin cell bodies are found in the cat but not in the rat. In particular the pain-related nuclei present a similar distribution of the peptide in the two species; however, met-enkephalin-containing cell bodies are much more numerous in the cat than in the rat (notably in the reticular formation). Similar types of met-enkephalin innervation occur in the dorsal and intermediate grey of the spinal cord and of the caudal trigeminal nucleus supporting further that the functional organizations of these regions are closely related.

US$29.90

PMID: 6365253


Other references

Reis, H.E.; Cremer, W.; Heimsoth, V.H.; Wetter, O.; Eitelhuber, U., 1974: Further studies on the cellular defense mechanism in patients with terminal kidney failure and on treatment using intermittent hemodialysis. Verhandlungen der Deutschen Gesellschaft für Innere Medizin 80: 749-752

D'arrigo, P.; Servi, S., 1997: Using phospholipases for phospholipid modification. Phospholipases, which modify phospholipids in natural membranes, are available from different sources for application in biocatalysis. All sites of phospholipids can be selectively modified by enzymatic catalysis. By coupling the phospholipase act...

Tan, B.K.; Adya, R.; Farhatullah, S.; Lewandowski, K.C.; O'Hare, P.; Lehnert, H.; Randeva, H.S., 2008: Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose. OBJECTIVE - Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that plasma omentin-1 levels decrease with obesity. Currently, no data exist on the relative expression and regulation of om...

Bertilsson, S.; Stepanauskas, R.; Cuadros Hansson, R.; Graneli, W.; Wikner, J.; Tranvik, L., 1999: Photochemically induced changes in bioavailable carbon and nitrogen pools in a boreal watershed. In several recent studies, a net stimulation of bacterial growth has been demonstrated after exposing humic surface waters to solar radiation or artificial ultraviolet radiation. This stimulation has been attributed to a photochemical release of b...

Turnipseed, W.D.; Sproat, I.A., 1992: A preliminary experience with use of magnetic resonance angiography in assessment of failing lower extremity bypass grafts. This study evaluates the ability of magnetic resonance angiography (MRA) to detect hemodynamic failure of lower extremity bypass grafts and questions whether conventional angiography is necessary for this purpose. A prospective surveillance protoc...

Evans C.H., 1985: Effects of cobalt on the production of collagenase prostaglandin e 2 and bone resorption. Orthopaedic Transactions: 551-552

Yancey T.E.; Stevens C.H.; Hanger R.A., 1988: Geographic placement of the eastern Klamath Terrane in the Pacific Ocean basin during the Early Permian. Abstracts with Programs - Geological Society of America 20(7): 308

Sharma, S.; Kanwar, S.S., 2015: Organic solvent tolerant lipases and applications. Lipases are a group of enzymes naturally endowed with the property of performing reactions in aqueous as well as organic solvents. The esterification reactions using lipase(s) could be performed in water-restricted organic media as organic solvent...

Waymouth, H.E.; Zoutman, D.E.; Towheed, T.E., 2014: Chikungunya-related arthritis: case report and review of the literature. Chikungunya fever often presents with severe arthritis/arthralgias, high fever, myalgias, headache, and maculopapular rash (Chow et al., 2011 [1]; Das et al., 2010 [2]; Mizuno et al., 2011 [3]; Powers, 2010 [4]; Sissoko et al., 2010 [5]; Staples e...

Cerezo González, L.; Sánchez, E.; Villarán, F.; Sobrino Daza, N.; Alberich Hidalgo, F.; Fernández Alvarez, A., 1976: Accidental injection of air into the left coronary artery. Revista Clinica Espanola 140(1): 83-85