+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Quantification without purification of blood and tissue adenosine by radioimmunoassay

, : Quantification without purification of blood and tissue adenosine by radioimmunoassay. Analytical Biochemistry 135(1): 156-164

Highly specific anti-adenosine antibodies were produced in rabbits by the injection of N6-carboxymethyl adenosine-methylated serum albumin conjugates. They were used to develop a radioimmunoassay allowing the quantitation of adenosine in the range 0.1-10 pmol per sample. Inosine did not interfere except at 300 times higher concentrations, while AMP (ATP) did not displace the [3H]adenosine tracer even at 10(5) (10(6) ) times higher amounts. Due to the high specificity of the anti-adenosine antibodies, determination of blood and tissue adenosine levels could be performed directly from perchloric acid extracts. Values for human peripheral venous blood from various donors obtained with this procedure varied between 46 and 148 pmol/ml blood. The procedure was also applied to HeLa cultures with low and high intracellular adenosine. The reliability of the method was demonstrated by comparative analyses using HPLC purification of adenosine prior to the radioimmunoassay.


PMID: 6670736

Other references

Svetlakov, M.I., 1959: Errors in medico-military otolaryngological expert examination. Voenno-Meditsinskii Zhurnal 2(5): 41-43

Jia Li; Yang JiaRong; Y.Z.eng; L.S.iYuan, 2007: Studies on differentiation of Venturia inaequalis of apple in pathogenicity. In order to explicit the pathogenicity differentiation of Venturia inaequalis in fields, three apple cultivars were individually inoculated with 57 representative single spore isolates of Venturia inaequalis collected respectively from different a...

Rose, B.R.; Thompson, C.H.; Jiang, X.M.; Tattersall, M.H.; Elliott, P.M.; Dalrymple, C.; Cossart, Y.E., 1994: Detection of human papillomavirus type 16 E6/E7 transcripts in histologically cancer-free pelvic lymph nodes of patients with cervical carcinoma. Human papillomavirus (HPV) 16 E6/E7 mRNAs have been detected in paraffin-embedded sections of histologically cancer-free pelvic lymph nodes from four of six patients with HPV 16-associated cervical cancer. The cDNA obtained from the viral mRNA by...

Anonymous, 1968: London School of Hygiene and Tropical Medicine. Report on the work of the School, 1967-68. The extent to which Toxocara infections have spread from animal to man in Britain and other countries is under investigation, and a skin sensitivity test has been devised and standardized, together with the development of a fluorescent antibody te...

Woodard Gary, 1987: Problems with hydrogeologic assessments in fractured carbonate terrains. Annual Meeting - Association of Engineering Geologists 30: 58

Kastan, M.B.; Slamon, D.J.; Civin, C.I., 1989: Expression of protooncogene c-myb in normal human hematopoietic cells. Expression of the protooncogene, c-myb, in various subpopulations of normal human hematopoietic cells was characterized. Cells expressing the immature cell surface marker, CD34 (My10), were isolated by immune adherence with the "panning"...

Yu, H.K.; Wu, H.J.; Lei, L.; Xu, W.Z.; Gao, L.X., 1989: A preliminary study on immune complexes in myasthenia gravis. The levels of circulating immune complex from 43 patients with myasthenia gravis (MG) and 50 normal controls were detected by using ELISA based on bovine conglutinin. The levels of circulating immune complex in MG patients were higher than those i...

Bansky, G., 1986: Extraintestinal manifestations in chronic inflammatory intestinal diseases. Schweizerische Rundschau für Medizin Praxis 75(11): 303-310

Rahier, Alain, 2001: Deuterated D7-cholesterol analogues as mechanistic probes for wild-type and mutated D7-sterol-C5(6)-desaturase. Deuterium-labeled 5a-cholest-7-en-3b-ol (1) bearing one or two deuteriums at the C-5a and (or) C-6a positions was synthesized in high isotopic and chiral purity. These compounds were used as substrates with the microsomal wild-type Zea mays and re...

Pugachev A.G.; Ankudinov M.V.; Mokhov O.I., 1991: Roentgenoendovascular study of children with varicocele. 60 patients with varicocele first diagnosed and 38 with postoperative recurrences of varicocele were examined with the use of digitalis subtraction renal and testicular phlebography as an optional diagnostic method. In the patients' group wit...