geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Quantification, characterisation and safety testing of foot-and-mouth disease virus antigens eluted from alhydrogel vaccines


, : Quantification, characterisation and safety testing of foot-and-mouth disease virus antigens eluted from alhydrogel vaccines. Developments in Biological Standardization 50: 277-283

Different methods for the elution and the concentration of FMDV-antigen from formaldehyde-inactivated alhydrogel vaccines were compared. With locally produced vaccines the best results were obtained if the antigen was eluted with potassium phosphate buffer and the concentration was performed by ultrafiltration. Final recoveries of 50% of the 140-S antigen could be obtained. For innocuity control 1-3 mg of 140-S antigen was eluted from 21 vaccine. SDS-polyacrylamide gel electrophoresis and iso-electric focusing patterns of eluted antigen will be discussed.

US$29.90

PMID: 6176487


Other references

Grove, J.R.; Prapavessis, H., 1992: Preliminary evidence for the reliability and validity of an abbreviated profile of mood states. Schacham (1983) developed a short version of the Profile of Mood States (POMS; McNair, Lorr, & Droppleman, 1971) for use with hospital patients. The present study examined the psychometric properties of a slightly modified form of Schacham's...

Soderpalm, B.; Svensson, A.I., 1999: Naloxone reverses disinhibitory/aggressive behavior in 5,7-DHT-lesioned rats; involvement of GABAA receptor blockade?. Effective medical treatment for impulsive aggression and several impulse control disorders is needed. Disinhibited, impulsive behavior of e.g. murderers, arsonists, suicidal patients, and patients suffering from antisocial personality or substance...

Barone, V.; Boilleau, C.; Cacelli, I.; Ferretti, A.; Monti, S.; Prampolini, G., 2013: Structure-Properties Relationships in Triplet Ground State Organic Diradicals: A Computational Study. A fast and efficient computational protocol, devised for the accurate calculation of singlet-triplet magnetic splittings in organic diradicals, is here applied to several promising organic magnets, recently considered in the literature. The very g...

Dusetti, N.J.; Mallo, G.; Dagorn, J.C.; Iovanna, J.L., 1994: Serum from rats with acute pancreatitis induces expression of the PAP mRNA in the pancreatic acinar cell line AR-42J. PAP is a pancreatic secretory protein expressed in the pancreas during the acute phase of pancreatitis. We have investigated the effect of the serum from rats with acute pancreatitis (SAP) on the expression of the PAP mRNA in AR-42J cells. PAP mRN...

Alfaro, B., 1992: Levels of freedom felt when participating in some recreational activities. From a very early stage all people face choices and decisions between courses of action, and have various degrees of freedom in making those decisions. The article offers an analysis of the literature covering this freedom of choice and then prese...

Roy, C.S., 1881: The Elastic Properties of the Arterial Wall. Journal of Physiology 3(2): 125-159

Kir'yalov, N.P., 1940: Essential oil of milfoil, Achillea micrantha M B. Essential oil of A. micrantha, obtained from flowers by steam-distn., had d2020 0.9288, n D18 1.4667, [alpha]D18 [long dash]11.23[degree], acid no. 2.41, ester no. 20.12, ester no. after acetylation 50.9. It contained cineole 63, a-terpineol about...

Elena, M.; Florica, Z., 1972: Citeva cazuri de intersexualitate spontana la Rana ridibunda. Analele Univ Bucuresti (Biol Anim): 21: 33-37

Pallarito, K., 1999: OK, we're not-for-profit, but ... show us the money!. For-profits aren't the only ones who like to deal. As recent transactions show, small fortunes can be had in merger and affiliation talks with not-for-profit healthcare systems. Although rarely publicized, it isn't uncommon to find expli...

Reinthaller, A., 1992: Diagnosis of benign and malignant vulvar changes. Gynakologisch-Geburtshilfliche Rundschau 32 Suppl 1: 11-13