+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Quantitative immunocytochemistry of corticotropin-releasing factor (CRF). Studies on nonbiological models and on hypothalamic tissues of rats after hypophysectomy, adrenalectomy and dexamethasone treatment

, : Quantitative immunocytochemistry of corticotropin-releasing factor (CRF). Studies on nonbiological models and on hypothalamic tissues of rats after hypophysectomy, adrenalectomy and dexamethasone treatment. Brain Research 293(1): 111-118

Indirect immunofluorescence cytochemistry of ovine corticotropin releasing factor (oCRF) was performed by use of an antiserum raised against a conjugate of oCRF and bovine thyroglobulin. The staining intensity was quantitated by use of an automated microfluorimeter. In cryostat sections of formaldehyde fixed oCRF containing gelatine models, the staining intensity was dependent on the concentration of oCRF (1-100 microM) added to the gel. Immunoinhibition experiments showed that oCRF induced identical concentration-dependent (0.001-1 microM) quenching of the immunostaining of oCRF containing models and rat median eminence (ME) preparations. Comparison of immunoinhibition of oCRF and ME extracts indicates that approximately 1.5 ng of CRF immunoreactivity (CRFi) is present in the ME of intact adult male Wistar rats. In the hypothalamus of rats, the majority of CRFi nerve fibers are localized in the external zone of the median eminence, whereas a large population of CRFi cell bodies is present in the paraventricular nucleus. Manipulations of the pituitary-adrenal system result in changes in the distribution of CRFi in these neurons. One week after extirpation of the adrenals or of the pituitary gland, the CRFi in the ME was reduced to 32 +/- 3% and 48 +/- 6% respectively. This decrease in CRFi in the median eminence can be largely prevented by treatment of rats with dexamethasone in doses that effectively reduce plasma ACTH and corticosterone levels. In contrast to intact rats, CRFi cell bodies can be visualized in the paraventricular nucleus of non-colchicine-treated rats after adrenalectomy or hypophysectomy. These data support the view that the hypothalamic CRFi neurons play a central role in the control of pituitary-adrenal activity.


PMID: 6367890

Other references

Kurt A.M.; Unni K.K.; Mcleod R.A.; Pritchard D.J., 1989: Intraosseous well differentiated osteosarcoma clinicopathologic study of 80 cases. Laboratory Investigation 60(1): 50A

Rongone, E.L.; Bocklage, B.C.; Strength, D.R.; Doisy, E.A., 1957: Hydrolysis of steroid acetates by bovine albumin. The C-21 acetates of cortisone, cortisol, deoxycorticosterone, 11-dehydrocorticosterone, and estrone-3-acetate were completely hydrolyzed by preparations of bovine blood albumin at pH 6.8 and 35[degree]. The identity of the isolated products from...

Knodt, C.B.; Petersen, W.E., 1946: Studies of the carbohydrate metabolism of mammary gland tissue in vitro. 3. Glycogen as an intermediary in the formation of lactose. In the perfused bovine mammary gland, the administration of 120 to 160 units of insulin markedly reduced the percentage of lactose in the milk and caused an accumulation of glycogen in the tissue, which was due apparently to blocking of glyeogenol...

King, BR., 1993: The status of Queensland seabirds. Corella. July; 173: 65-92

Ensink, K.; Robertson, B.A.; Zissis, C.; Leger, P.; de Jager, W., 1998: Conduct disorder among children in an informal settlement. Evaluation of an intervention programme. This study aimed to evaluate the effectiveness of a 12-week intervention programme for conduct-disordered boys aged 10-16 years. A descriptive study comparing a group of boys who participated in an intervention programme with a non-participant gro...

Acchiardo, S.R.; Moore, L.; Smith, S.O.; Burk, L.B.; Smith, S.J.; Will, K., 1995: Measurements of urea rebound in hemodialysis patients and their implications. Journal of the American Society of Nephrology 6(3): 591

Stolbkov, I.K., 1976: Effect of centrifugal force on nystagmus during angular accelerations. Fiziologicheskii Zhurnal SSSR Imeni I. M. Sechenova 62(10): 1447-1451

Besler, B.A.; Sondergaard, R.E.; Müller, R.; Stok, K.S., 2016: Reproducibility of compartmental subchondral bone morphometry in the mouse tibiofemoral joint. Evidence suggests that subchondral bone can be used as a predictor for the onset of osteoarthritis. As such, there is a need to accurately and reproducibly quantify subchondral bone in areas where osteoarthritis develops. In this paper, we present...

Taube, M., 1964: The Coming Of Age Of Information Technology. A discussion of the distinction between a scientific discipline and a professional activity and of the reasons why "information technology" is a professional and not a scientific activity. The application of computers to the improvement...

Junqueira, LCU., 1972: Effect of vinblastine, colchicine, sodium, potassium and adrenalin on pigment migration of melanophores in ten species of teleosts. Anais Acad Bras Cienc, 441: 167-169