+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Rapid determination of minimum inhibitory concentrations of antimicrobial agents by regression analysis of light scattering data

, : Rapid determination of minimum inhibitory concentrations of antimicrobial agents by regression analysis of light scattering data. Antimicrobial Agents and ChemoTherapy 17(5): 813-823

A novel, rapid, automated method for determining the minimum inhibitory concentrations of antimicrobial drugs within ranges acceptable for therapeutic application is described and validated in this report. By employing a simple modification of the Autobac photometer currently in use and in optically clear, modified Mueller-Hinton broth, the method utilizes forward light scattering data measured in the presence of two concentrations of the antimicrobial agent to compute the minimum inhibitory concentrations. Empirically derived regression equations which simultaneously use scattering data from two drug concentrations are employed in this computation, rather than simple breakpoint analysis in which the light scattering measured in the presence of each drug concentration is referred to a threshold level. The minimum inhibitory concentrations obtained with this new method were highly reproducible and, as shown by side by side comparisons were in excellent agreement with the minimum inhibitory cencentrations obtained with the International Collaborative Study broth dilution method.


PMID: 7396468

Other references

Caro L.H.P.; Plomp P.J.A.M.; Leverve X.M.; Meijer A.J., 1989: A combination of intracellular leucine with either glutamate or aspartate inhibits autophagic proteolysis in isolated rat hepatocytes. It has been shown previously that the inhibition of autophagic proteolysis in liver by a physiological mixture of amino acids can be mimicked completely by addition of leucine in combination with alanine [Leverve, X. M., Caro, L. H. P., Plomp, P....

Sherman, C., 1993: Behind closed doors: therapist-client sex. Psychology Today 26(3): 64-72

Damiens, E.; Meijer, L., 2000: Chemical inhibitors of cyclic-dependent kinases: preclinical and clinical study. In the past decade, the use of a large variety of cellular models and cell biology, biochemistry and molecular biology techniques has led to the discovery of key proteins that are intimately involved in the regulation of tumor growth. In particula...

Gallerani, M.; Manfredini, R., 2013: Seasonal variation in the occurrence of delirium in patients admitted to medical units of a general hospital in Italy. Delirium syndrome is common in the hospitalised population. However, data on its aetiological factors are scarce. Clinical observations suggest a relationship between delirium occurrence and seasons. The aim of study was to determine whether a sea...

Gopalan, C., 1961: Maternal and infant nutrition in underdeveloped countries. Studies on over 300 pregnant women indicate that inadequate diets of the mothers result in poor condition of both mothers and babies. Mothers gain little weight during pregnancy, have a high incidence of anemia and B-complex deficiencies, have low...

Broadfield, L.; Lee, P.M.; Seelinger, P.M.; Heffron, D.T.; Dibelka, A.W.; Rodenrys, J.J.; Feeney, R.J.; Calabro, J.M.; Conrad, W., 2000: Method and apparatus for the dispensing of drugs. A system and apparatus are described in which modular receptacles are filled and transported to automatic dispensing machines for later retrieval and distribution. A system of the present invention includes the loading, refilling, and replacement...

Tachibana, H.; Meyer, J.S.; Kitagawa, Y.; Rogers, R.L.; Okayasu, H.; Mortel, K.F., 1984: Effects of aging on cerebral blood flow in dementia. Regional cerebral blood flow values were measured utilizing the 133Xe inhalation method in patients with multi-infarct dementia (MID) (n = 22, age 67.4 +/- 9.8 years), in patients with senile dementia of Alzheimer type (SDAT) (n = 36, age 63.8 +/-...

Cameron, C.E., 1953: Fundamentals of a membership campaign. Nursing Outlook 1(10): 560-563

Wessels, J.P.H.; Kennedy, J.P.B., 1969: A study of pre-natal and post-natal growth in Pekin ducks. The h2 of 8-wk body weight was calculated, based on parent-offspring regression and half-sib analysis. 56 and 196 parents were involved.

Mazaris, A.D.; Broder, B.; Matsinos, Y.G., 2006: An individual based model of a sea turtle population to analyze effects of age dependent mortality. Effective conservation of wildlife species relies on our ability to recognize interactions among critical life stages, leading to the design of the appropriate protection measures. We developed an individual based model to analyze the variability...