geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Recombinant human immunodeficiency virus type-1 (HIV-1) Tat protein sequentially up-regulates IL-6 and TGF-beta 1 mRNA expression and protein synthesis in peripheral blood monocytes


, : Recombinant human immunodeficiency virus type-1 (HIV-1) Tat protein sequentially up-regulates IL-6 and TGF-beta 1 mRNA expression and protein synthesis in peripheral blood monocytes. British Journal of Haematology 88(2): 261-267

In this study we evaluated the effect of human immunodeficiency virus type 1 (HIV-1) recombinant Tat protein on mRNA expression and protein synthesis of two inflammatory cytokines-interleukin-6 (IL-6) and transforming growth factor-beta 1 (TGF-beta 1)-by peripheral blood (PB) monocytes. Whereas maximal levels of IL-6 protein were recovered in PB monocyte culture supernatants after 24-48 h from the addition of 1 micrograms/ml of recombinant Tat, TGF-beta 1 showed a slower and progressive increase, reaching maximal levels only after 72-96 h of culture. Consistently, the analysis of the steady-state levels of mRNA showed a sharp increase of IL-6 mRNA expression after 24h of culture, with a slow decline thereafter. On the other hand, TGF-beta 1 mRNA expression showed a slow increase only after 72-96 h of culture. Moreover, IL-6 appeared involved in the up-regulation of TGF-beta 1, because the addition of a neutralizing anti-IL-6 antibody to Tat-treated PB monocyte cultures significantly reduced the amounts of TGF-beta 1 recovered in the culture supernatants after 96 h. The present demonstration that HIV-1 Tat protein directly up-regulates IL-6 expression and stimulates TGF-beta 1 production both directly and indirectly, through early IL-6 production, could have important implications in the pathogenesis of HIV-1 disease.

US$29.90

PMID: 7803268


Other references

Brambilla, R.; Dvoriantchikova, G.; Barakat, D.; Ivanov, D.; Bethea, J.R.; Shestopalov, V.I., 2013: Transgenic inhibition of astroglial NF-κB protects from optic nerve damage and retinal ganglion cell loss in experimental optic neuritis. Optic neuritis is an acute, demyelinating neuropathy of the optic nerve often representing the first appreciable symptom of multiple sclerosis. Wallerian degeneration of irreversibly damaged optic nerve axons leads to death of retinal ganglion cel...

Takahashi H., 1974: Genetical and physiological analysis of interspecific incompatibility between nicotiana alata and nicotiana langsdorffii. Japanese Journal of Genetics 49(4): 247-256

Pucci, G.N.; Pucci, H., 2004: Variation in the composition of Rhodococcus rodochrous GNP-OHP-38r cell membrane fatty acids in response to temperature and salinity. The members of the genus Rhodococcus are frequent and abundant inhabitants of polluted areas with hydrocarbons and they resist the salinity present in the central Patagonia, Argentina. This genus has good capacity to eliminate pollution produced b...

Tomlinson, J.; Faithfull, E1; Flewett, T.1; Beards, G., 1982: Isolation of infective tomato bushy stunt virus after passage through the human alimentary tract Plant virus vectors. Nature 22, 300(5893): 637-638

Toruniowa, B., 1964: Death Following Acth Administration. Polski Tygodnik Lekarski 19: 1860-1861

Joles, J.A.; Kriz, W.; Janssen, U.; Koomans, H.A.; Floege, J., 1997: Early signs of podocyte, but not mesangial cell, injury in hyperlipidemic female analbuminemic rats. Journal of the American Society of Nephrology 9(PROGRAM AND ABSTR ISSUE): 619A, Sept

Awunor, O.; Berger, D.; Kirisits, C., 2016: A multicenter study to quantify systematic variations and associated uncertainties in source positioning with commonly used HDR afterloaders and ring applicators for the treatment of cervical carcinomas. The reconstruction of radiation source position in the treatment planning system is a key part of the applicator reconstruction process in high dose rate (HDR) brachytherapy treatment of cervical carcinomas. The steep dose gradients, of as much as...

Sawicki, K., 1981: Role of the bill of costs and results in socialist economy Poland.1. Spoleczno gospodarcze funkcje nauki: 9

Tangnu S.K.; Blanch H.W.; Wilke C.R., 1981: Enhanced production of cellulase hemi cellulase and beta glucosidase by trichoderma reesei rut c 30. The production of cellulases and hemicellulases was studied with T. reesei Rut C-30. This organism produced, together with high cellulase activities, considerable amounts of xylanases and .beta.-glucosidase. Three cellulose concentrations (1, 2.5...

Montini, T.; Rossi, O.; Dacha, U., 1955: Contractile activity of the smooth muscle of the intestine following artificial hypothermia. Archivio "e. Maragliano" di Patologia E Clinica 10(2): 421-430