geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Sedimentation properties of native and proteolysed preparations of ox glutamate dehydrogenase


, : Sedimentation properties of native and proteolysed preparations of ox glutamate dehydrogenase. Biochemical Journal 199(1): 235-238

The concentration-dependent aggregation behaviour of purified ox liver and brain glutamate dehydrogenase preparations was compared with that of commercially-obtained preparations of the liver enzyme, which have recently been shown to have suffered proteolytic cleavage. Although there were no significant differences in these effects, the presence of 3 mM-GTP and 3 mM-NADH had markedly different effects on the two types of preparation. In this situation, at higher protein concentrations the commercially obtained preparations existed in a higher degree of aggregation than those which had not suffered proteolysis. Studies of the effects of GTP and NADH concentrations on the sedimentation coefficients at a fixed enzyme concentration suggested these effects to be largely due to differences in the affinities of the two preparations for nucleotides.

US$29.90

PMID: 7337705


Other references

Chacalo, A.; Pineau, M., 1991: Environmental problems and urban trees in Mexico City. Choice of species, transplantation, tree reservations and maintenance carried out in Mexico City are analysed, in the context of the environmental stresses (pollution, erosion, population growth) present. It is recommended that an inventory of urb...

Zhuk, Rk, 1974: Possible replacement of animal p. Ptakhivnitstvo 7 37-41

Orlandini, S.; Pasquini, B.; Del Bubba, M.; Pinzauti, S.; Furlanetto, S., 2015: Quality by design in the chiral separation strategy for the determination of enantiomeric impurities: development of a capillary electrophoresis method based on dual cyclodextrin systems for the analysis of levosulpiride. Quality by design (QbD) concepts, in accordance with International Conference on Harmonisation Pharmaceutical Development guideline Q8(R2), represent an innovative strategy for the development of analytical methods. In this paper QbD principles ha...

Naranjo, A.Amezquita.; Triviño, M.Lucia.; Jaramillo, A.; Betancourth, M.; Botero, J.Enrique., 2006: Changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement. Dental plaque is organized in a biofilm complex that provides protection and nutrients for periodontopathic bacteria. Several factors can affect microbial colonization, including restorations and orthodontic brackets. The aim of this study was to...

van Delft, K.; Schwertner-Tiepelmann, N.; Thakar, R.; Sultan, A.H., 2014: Recruitment of pregnant women in research. The aim was to identify factors that could influence recruitment in a prospective longitudinal study involving pregnant women. A total of 269 nulliparous women were enrolled for a prospective longitudinal study, to establish the prevalence of leva...

Cheal, N.E.; McKnight-Eily, L.; Weber, M.Kate., 2015: Alcohol Screening and Brief Intervention: A Clinical Solution to a Vital Public Health Issue. American Nurse Today 9(9): 34-35

Klinke, F.; Liss, E.; Palme, G., 1965: 3H-endoxan: distribution kinetics and DNA binding. Nuclear-Medizin 2: 47-53

Gross, A.T.H., 1972: Stress wheel - a planting design for forage crop introductions. Some advantages and disadvantages of the stress-wheel planting design for evaluating new and introduced forage varieties are discussed. In such a system plants are grown in single or double rows radiating from a centre point, where the between-row...

Chandrasekaran, V.; Gopi, P.G.; Santha, T.; Subramani, R.; Narayanan, P.R., 2007: Status of re-registered patients for tuberculosis treatment under DOTS programme. To assess the proportion of patients re-registered after default, failure or successful treatment, completion and evaluate their treatment outcome. Tuberculosis patients diagnosed were registered for treatment under DOTS in rural area, South India...

O'Neill, G.M.; Donovan, G.R.; Baldo, B.A., 1994: Cloning and characterization of a major allergen of the house dust mite, Dermatophagoides pteronyssinus, homologous with glutathione S-transferase. A major allergen of the house dust mite, Dermatophagoides pteronyssinus, has been identified and characterized from a lambda gt11 cDNA library of the mite. IgE antibodies from the sera of allergic patients that recognise the cloned polypeptide bin...