+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Separation of radioiodinated angiotensins by chromatofocusing in minicolumns

, : Separation of radioiodinated angiotensins by chromatofocusing in minicolumns. Analytical Biochemistry 138(2): 303-308

Angiotensins I, II, and III (AI, AII, AIII) and Saralasin (Sar1-Ala8-AII) were labeled with 125I and separated from the nonlabeled forms on minicolumns (a Pasteur pipet ) of chromatofocusing medium. At low ionic strength, 125I-labeled angiotensins could be eluted with Polybuffer or a piperazine-histidine buffer at their approximate isoelectric points, while nonlabeled angiotensins remained adsorbed to the column and required 1 mol X liter-1 NaCl for elution. The 125I-labeled angiotensins prepared by this method were bound by antibodies (AI) and adrenal receptors (AII, Saralasin) to an extent similar to angiotensins prepared by DEAE-Sephadex A-25 chromatography. This new method of preparing radioiodinated angiotensins is rapid (15 min), inexpensive, and requires no fraction-collecting equipment.


PMID: 6742406

Other references

Morgan, J.H.C., 2003: New approaches to training general practitioners. Postgraduate Medical Journal 79(930): 187-188

Thielemann, H.; Grahneis, H., 1982: Thin-layer chromatographic analysis of toxaphene in model solutions and surface waters of the Halle region. Zeitschrift für die Gesamte Hygiene und Ihre Grenzgebiete 28(5): 324-328

D.M.rzi S.; Morini V., 1972: The use of meglumine indomethacinate in the treatment of pain in neoplastic affections. Clinica Terapeutica 62(2): 175-180

Berry, E.M., 2001: Drug therapy for management of obesity. Lancet 357(9264): 1287; Author Reply 1288-1287; Author Reply 1288

Antonelli, M.A., 1994: Practicing physical evaluation skills on community volunteers. Academic Medicine 69(3): 214-215

Mihail, J.D.; Champaco, E.R., 1993: Diseases of Amaranthus spp. caused by Pythium aphanidermatum and Macrophomina phaseolina. Experimental plantings of several species and crosses of A. were monitored during the 1990-92 growing seasons to identify diseases that might limit commercial production of grain amaranth in Missouri. Two diseases, previously unreported on the A....

Schroeck, F.; Arroyo de Prada, N.; Sperl, S.; Schmitt, M.; Viktor, M., 2002: Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin (Vn): mapping the binding sites on PAI-1 and Vn. The serpin plasminogen activator inhibitor type-1 (PAI-1), as the primary physiological inhibitor of both urokinase-type (uPA) and tissue-type (tPA) plasminogen activator, plays an important role in the regulation of the fibrinolytic system as wel...

D.I.Vvedensky., 1936: On the methodics of breeding white cabbage for earliness. Early maturity is a feature in which nearly all varieties, being cross-pollinators, are to a greater or lesser extent heterozygous. The best methods so far proposed for determining time of maturity are somewhat arbitrary, by visual observation, to...

Wardle, E.N., 1974: Reticuloendothelial clearance studies in the course of horse serum induced nephritis. British Journal of Experimental Pathology 55(2): 149-152

Wu, Q.; Yin, H., 2011: N-Hy-droxy-pyridine-4-carboxamide. The title compound, C(6)H(6)N(2)O(2), is approximately planar with an r.m.s. deviation for the non-H atoms of 0.052 Å. In the crystal, a two-dimensional array in the bc plane is stabilized by O-H⋯N and N-H⋯O hydrogen bonds.