geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Synthesis of mitochondrial protein in the flight muscles of the Colorado beetle. Differentiation in vivo between extra- and intra-mitochondrial contributions to the amino acid incorporation into mitochondrial protein


, : Synthesis of mitochondrial protein in the flight muscles of the Colorado beetle. Differentiation in vivo between extra- and intra-mitochondrial contributions to the amino acid incorporation into mitochondrial protein. Biochemical Journal 136(3): 795-802

By using cycloheximide, an inhibitor of cytoplasmic protein synthesis, conditions were investigated to estimate in vivo the extra- and intra-mitochondrial contributions to the synthesis of organelle protein in the flight muscles of Colorado beetles. With 4-day-old beetles about 15% of the [(14)C]leucine incorporation into mitochondrial protein is resistant to the action of cycloheximide. The incorporation into cytosol protein is inhibited by more than 99.5% with cycloheximide. During the first hour after precursor administration the incorporation into mitochondrial protein proceeds, in both the presence and the absence of cycloheximide, at a more-or-less linear rate with time. The cycloheximide-resistant amino acid incorporation is sensitive to the inhibitor of mitochondrial protein synthesis, chloramphenicol. The uncertainties inherent in the use of cycloheximide were discussed in arriving at the conclusion that about 15% of the mitochondrial protein is formed inside the organelle.

US$29.90

PMID: 4780702


Other references

Vargas-Albores, F.Y.piz-Plascencia, G., 2000: Beta glucan binding protein and its role in shrimp immune response. Despite their relatively short life and assumed lesser complexity, crustaceans have mechanisms to detect foreign matter. In particular, they appear to recognize common characteristics present in bacteria and fungi, such as lipopolysaccharides (LPS...

Ryu, J.K.; Jantaratnotai, N.; Serrano-Perez, M.C.; McGeer, P.L.; McLarnon, J.G., 2011: Block of purinergic P2X7R inhibits tumor growth in a C6 glioma brain tumor animal model. We examined the expression and pharmacological modulation of the purinergic receptor P2X7R in a C6 glioma model. Intrastriatal injection of C6 cells induced a time-dependent growth of tumor; at 2 weeks postinjection immunohistochemical analysis de...

Winkler, A.; Wagener, H.H., 1975: Further investigational results with the dermatometer according to bingmer. Further results of investigations using the dermatometer according to Bingmer for the determination of the actual moisture content of superficial skin layers are presented. The investigations are divided into in-vitro tests, tests on excised skin...

Rowe, J.M.; Crison, J.R.; Carragher, T.J.; Vatsaraj, N.; McCann, R.J.; Nikfar, F., 2014: Mechanistic insights into the scale-up of the roller compaction process: a practical and dimensionless approach. Although the roller compaction process appears simple, efforts to quantitatively model the process have proven challenging because of complex material behavior in the feeding and compaction zones. To date, implementation of roller compaction model...

Sanders, C.L.; Lundgren, D.L., 1995: Pulmonary carcinogenesis in the F344 and Wistar rat after inhalation of plutonium dioxide. Pulmonary carcinogenesis was compared in female F344 and Wistar rats after inhalation of high-fired 239PuO2. Plutonium particle aggregation, as determined by quantitative light and scanning electron microscopic autoradiography, was greater for the...

Cantoni, C.; Provana, L., 1993: Food labeling The new law. Rivista di Scienza dell'Alimentazione 22(3): 379-388

Anonymous, 1971: Problems in research on plant viruses and in the study of phytopathogenic micro-organisms. This collection of papers presented at a symposium held in Aschersleben in June, 1970, includes the following of horticultural interest: The results of eleven years' studies on viruses and virus diseases of ornamental, forest and wild woody p...

Ishihara, S., 1973: Amount of food-intake and digestive speed by the lizard.Tachydromus tachydromoides. Acta herpet: 28.

Wang, M.; Fu, Y.; Gao, C.; Jia, Y.; Huang, Y.; Liu, L.; Wang, X.; Wang, W.; Kong, W., 2016: Cartilage oligomeric matrix protein prevents vascular aging and vascular smooth muscle cells senescence. Aging-related vascular dysfunction contributes to cardiovascular morbidity and mortality. Cartilage oligomeric matrix protein (COMP), a vascular extracellular matrix protein, has been described as a negative regulatory factor for the vascular agin...

Matsuzaki, M.; Matsuda, Y.; Ikee, Y.; Takahashi, Y.; Sasaki, T.; Toma, Y.; Ishida, K.; Yorozu, T.; Kumada, T.; Kusukawa, R., 1981: Esophageal echocardiographic left ventricular anterolateral wall motion in normal subjects and patients with coronary artery disease. Esophageal echocardiography was developed for recording left ventricular anterolateral wall (LVAW) echocardiograms and was applied clinically to 14 normal subjects and 21 patients with coronary artery disease. LVAW echocardiograms were obtained sa...