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Transfer RNA chromatography on reversed phase five: effect of cadmium ion on a queuine-type tRNA


, : Transfer RNA chromatography on reversed phase five: effect of cadmium ion on a queuine-type tRNA. Biochemical and Biophysical Research Communications 123(3): 1027-1032

A sensitive method is described that detects an alteration in the structure of tRNA that is caused by cadmium but not by magnesium or zinc ions. The chromatographic system, RPC-5, separates Drosophila tyrosyl-tRNA into two fractions. These two isoacceptors differ by a single position in the anticodon where either a guanosine or queuine resides. Cadmium ions apparently interact with the tRNA and prevent the chromatographic separation. This is the first instance where cadmium is shown to cause a selective change in nucleic acid structure. The RPC-5 system seems to be uniquely useful in detecting such a change.

US$29.90

PMID: 6435622


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