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Under-hydroxylation of collagen synthesized by cultured fibroblasts

, : Under-hydroxylation of collagen synthesized by cultured fibroblasts. Comptes Rendus des Seances de L'academie des Sciences. Serie D, Sciences Naturelles 291(2): 211-213

The collagen secreted by dermal fibroblast cell cultures, previously labelled with 3H-proline, was extracted and purified by carboxymethyl cellulose. The ratio of hydroxyproline to proline showed that the collagen produced by cell cultures may be under-hydroxylated in comparison to the ratio observed in the types of collagen extracted from human skin. Then, it is concluded that it is not sufficient to determine only the hydroxyproline content for a collagen biosynthesis study in cell cultures. This low hydroxyproline content may explain the rapid catabolism of a part os newly synthetized collagen in vitro.


PMID: 6775829

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Cho, E.Ju.; Kim, S.Eun.; Suk, K.Tae.; An, J.; Jeong, S.Won.; Chung, W.Jin.; Kim, Y.Jun., 2017: Current status and strategies for hepatitis B control in Korea. Hepatitis B virus (HBV) infection is the most common cause of chronic liver diseases in Korea. After the introduction of the universal HBV vaccination program, the prevalence of hepatitis B surface antigen was markedly reduced, and Korea is now cl...

Bernal, E.W., 1988: Hysterectomy and autonomy. Theoretical Medicine 9(1): 73-88

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Höllerl, G.; Prexl, H.J., 1975: Gastroscopy -- a routine procedure in the investigation of diseases of the stomach. After a short review of the development of endoscopic investigations, the technique of gastroscopy is described and the indications for this procedure are pointed out. This procedure is of equal importance as radio-diagnostic measures in the routi...

Savov S.; Petkov Iv, 1972: Deep wells in the Tundzha faulted area. Geophysical surveys, tectonics, Bulgaria.

Kern, F., 1979: Clinical training in gastroenterology: a proposal for restoring balance. Gastroenterology 76(6): 1489-1492

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