+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Use of imprints for monoclonal antibody studies: suitability of air-dried preparations from lymphoid tissues with an immunohistochemical method

, : Use of imprints for monoclonal antibody studies: suitability of air-dried preparations from lymphoid tissues with an immunohistochemical method. American Journal of Clinical Pathology 79(4): 438-442

Air-dried imprint preparations are conveniently produced from human lymphoid samples without the special methods required for snap-freezing tissues or rendering them into suspensions. T-cells, T-cell subsets (helper and suppressor), and HLA-DR-positive cells (B-lymphocytes, monocytic-histiocytic cells) can be identified in such imprints by the use of commercially obtained mouse hybridoma antibodies with a simple two-step immunoperoxidase method. Direct nuclear morphologic correlation with surface determinants is achieved by this method. Immunoreactivity is retained only eight to 10 days in such air-dried preparations, and attempts to prolong reactivity have been unsuccessful so far.


PMID: 6220599

Other references

Smadja, N.-Veronique; Louvet, C.; Isnard, F.; Dutel, J.-Louis; Grange, M.-Jose; Varette, C.; Krulik, M., 1995: Cytogenetic study in multiple myeloma at diagnosis: Comparison of two techniques. Cytogenetic studies in multiple myeloma (MM) have been disappointing due to the low mitotic index of plasma cells. Recently the detection of clonal chromosomal abnormalities at diagnosis seemed to be improved by addition of cytokines (IL-6 and GM-...

Maksiutina, N.P.; Zinchenko, T.V.; Pasechnik, I.Kh.; Shmorgun, S.S.; El'-Kommos, M.I., 1977: New cholagogic drug preparation, "Flastapiol". Farmatsevtychnyi Zhurnal: 76-80

Steyn H.K., 1981: Coordination of the provision of health information a study of 4 resources. During the 1979 Health Year a program was undertaken in the Cape Province, South Africa, to coordinate the resources of 2 well-known national health information centers with the resources of 2 relatively unknown provincial health information resou...

Goodman I.; Hiatt R.B., 1978: Binding characteristics of coherin by thin layer gel chromatography. Federation Proceedings 37(6): 1349

Gridley, M.F., 1951: A modification of the silver impregnation method of staining reticular fibers. A technic is presented for a simple modification of the Ag impregnation of reticular fibers. Multiple slides may be stained at one time by pouring the solns. on and off the slides in a Coplin jar. A 0.5% aqueous soln. of periodic acid is used for...

Chigi, F., 1912: Catalogo della Collezione ornitologica romana. Bollettino della Societa Zoologica Italiana, 1 217-243

Jeffery, P.L.; Duncan, R.P.; Yeh, A.H.; Jaskolski, R.A.; Hammond, D.S.; Herington, A.C.; Chopin, L.K., 2004: Expression of the ghrelin axis in the mouse: an exon 4-deleted mouse proghrelin variant encodes a novel C terminal peptide. Ghrelin, an n-octanoylated 28-amino-acid peptide capable of inducing GH secretion and food intake in humans and rats, is the endogenous ligand for the GH secretagogue receptor (GHS-R). Here we describe the expression and tissue distribution of the...

Inghe, G., 1959: Cooperation of physicians with social service organizations. Social-Medicinsk Tidskrift 36(2): 43-54

Kahlenberg, C.A.; Patel, R.M.; Nair, R.; Deshmane, P.P.; Harnden, G.; Terry, M.A., 2015: Clinical and Biomechanical Evaluation of an All-Arthroscopic Suprapectoral Biceps Tenodesis. Pathology of the long head of the biceps (LHB) is a well-recognized cause of shoulder pain in the adult population and can be managed surgically with tenotomy or tenodesis. To compare the biomechanical strength of an all-arthroscopic biceps tenode...

Cammack, P.M.; Donahue, M.P.; Manoli, A., 2004: The bridge and barrel hoop plates as alternatives to external fixation techniques in the foot and ankle. External fixation is a useful tool for treating severe traumatic injuries to the foot and ankle. External fixation, however, does have associated complications, such as inadequate fixation and pin tract infections. Temporary bridge plating of the...